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Glyceraldehyde 3-Phosphate Assay试剂盒(Fluorometric) (ab273344)

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Example data
  • Example data
  • Example data

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Adherent cells, Suspension cells, Tissue

概述

  • 产品名称

    Glyceraldehyde 3-Phosphate Assay试剂盒(Fluorometric)

  • 检测方法

    Fluorescent

  • 样品类型

    Tissue, Adherent cells, Suspension cells

  • 检测类型

    Quantitative

  • 实验步骤

    Multiple steps standard assay

  • 产品概述

    Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (ab273344) is a robust assay for the measurement of Glyceraldehyde 3-Phosphate (GA3P) in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm. 

  • 说明

    This product is manufactured by BioVision, an Abcam company and was previously called K2018 Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric). K2018-100 is the same size as the 100 test size of ab273344.

    The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    Developer Mix V 1 vial
    Development Enzyme Mix VI 1 vial
    GA3P Assay Buffer 1 x 25ml
    Glyceraldehyde-3-Phosphate 1 x 200µl
    PicoProbe I 1 x 400µl

图片

  • Example data
    Example data

    Example data.

    Kinetics of enzymatic reaction to measure GA3P in rat muscle (608 ng) and mouse kidney (8.5 µg) lysate with and without GA3P Standard spikes.

  • Example data
    Example data

    Example data.

    Interpolation of kinetic data for mouse kidney lysate. Δ GA3P = (GA3P spiked – GA3P unspiked).

  • Example data
    Example data

    Example data.

    GA3P in rat muscle and mouse kidney lysates.

文献 (2)

发表研究结果有使用 ab273344？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab273344 被引用在 2 文献中.

  • Solier S  et al. A druggable copper-signalling pathway that drives inflammation. Nature 617:386-394 (2023). PubMed: 37100912
  • Huang X  et al. IL-6/STAT3 Axis Activates Glut5 to Regulate Fructose Metabolism and Tumorigenesis. Int J Biol Sci 18:3668-3675 (2022). PubMed: 35813468

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