JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB102527

Glutamate Dehydrogenase Activity Assay试剂盒

Glutamate Dehydrogenase Activity Assay Kit

Be the first to review this product! Submit a review

|

(27 Publications)

Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

查看别名

GLUD, GLUD1, GDH 1, GLUDP1, GLUD2, GDH 2

5 Images
Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)
  • FuncS

Lab

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured in various samples showing O.D.'s in time course

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)
  • FuncS

Supplier Data

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

NADH Standard Curve

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)
  • FuncS

Lab

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured in cell lysates showing activity (mU) per 106 cells tested

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)
  • FuncS

Lab

Functional Studies - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

GDH measured showing activity (mU) per mL of tested sample

Other - Glutamate Dehydrogenase Activity Assay Kit (AB102527)
  • Other

Supplier Data

Other - Glutamate Dehydrogenase Activity Assay Kit (AB102527)

Representative image of Glutamate Dehydrogenase Activity Assay Kit ab102527

Components shown from left to right :

- NADH Standard I

- Glutamate Solution

- Developer Solution III

- GDH Positive Control

- GDH Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

检测方法

Colorimetric

样品类型

Urine, Heparin Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

检测类型

Enzyme activity

灵敏度

> 0.01 mU/well

检测平台

Microplate reader

style
left-column

搭配使用产品

Assay Kits

AB105134

Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

  • 5
  • 2
Assay Kits

AB105135

Aspartate Aminotransferase Activity Assay Kit

Functional Studies - Aspartate Aminotransferase Activity Assay Kit (AB105135)

  • 5
  • 1
Assay Kits

AB83360

Ammonia Assay Kit

Functional Studies - Ammonia Assay Kit (AB83360)

  • 5
  • 2
Assay Kits

AB83389

Glutamate Assay Kit

Functional Studies - Glutamate Assay Kit (AB83389)

  • 4
  • 2
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.

Other Notes
This product was previously called K729 Biovision Glutamate Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102527-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"GDH Assay Buffer", "number":"AB102527-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"Glutamate Solution", "number":"AB102527-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB102527-CMP02", "productcode":"AB255098" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB102527-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"GDH Positive Control", "number":"AB102527-CMP03", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate dehydrogenase (GDH) also known as GDH1 or GLUD1 plays a critical role in ammonia metabolism by catalyzing the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme with a molecular mass of approximately 55 kDa is predominantly expressed in the liver kidney brain and pancreas. It localizes to the mitochondrial matrix indicating its importance in cellular energy production. Researchers often study GDH through various assays like the GDH assay to understand its enzymatic activities and kinetic properties.
Biological function summary

This enzyme facilitates the integration of amino acid and carbohydrate metabolism by regulating glutamate levels and influencing the tricarboxylic acid (TCA) cycle. GDH does not form part of a larger complex but acts independently. Its activity is essential for maintaining the balance between energy supply and demand in cells impacting processes such as insulin secretion in pancreatic beta-cells. Inhibitors of GDH activity like some described by Ari Meneguzzi demonstrate its importance in metabolic regulation.

Pathways

GDH functions in the ammonia detoxification and urea cycle pathways. These pathways involve the conversion of glutamate ensuring efficient nitrogen removal from the body. Key proteins linked to GDH in these pathways include carbamoyl phosphate synthetase and ornithine transcarbamylase as they collectively manage nitrogen flow. GDH also intertwines with glutamine dehydrogenase highlighting its involvement in amino acid metabolism.

GDH is linked to hyperinsulinism-hyperammonemia (HI/HA) syndrome and hepatic encephalopathy. HI/HA syndrome occurs due to mutations in the GDH gene leading to inappropriate insulin secretion. Proteins such as GLUD1 are connected to GDH in the context of HI/HA. In hepatic encephalopathy altered GDH activity contributes to ammonia accumulation affecting brain function. Here GDH interactions with other mitochondrial proteins become important in disease progression.
style
left-column

产品实验方案

style
left-column

靶点信息

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
See full target information GLUD1

其他靶点

GLUD2
style
left-column

文献 (27)

Recent publications for all applications. Explore the full list and refine your search

NPJ biofilms and microbiomes 11:112 PubMed40541930

2025

A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis.

Applications

Unspecified application

Species

Unspecified reactive species

David Ranava,Stephen M Lander,Szu-Yu Kuan,Jonathan D Winkelman,Arthur Prindle,Mee-Ngan F Yap

Toxicology reports 14:102009 PubMed40226810

2025

Potential amelioration of liver function by low-dose tolvaptan in heart failure patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Mino,Kohei Hoshikawa,Takafumi Naito,Shunta Akutsu,Yumi Imoto,Emi Nakatsugawa,Masao Saotome,Yuichiro Maekawa,Junichi Kawakami

BMC infectious diseases 24:800 PubMed39118006

2024

New biomarkers for liver involvement by dengue infection in adult Vietnamese patients: a case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Nguyen Thi Cam Huong,Nguyen Phuong Hai,Chau Van Khanh,Mohamed Gomaa Kamel,Nguyen Van Vinh Chau,Nguyen Thanh Truong,Nguyen Thanh Vinh,Randa Elsheikh,Abdelrahman M Makram,Aya Elsheikh,Hiep Nguyen Canh,Somia Iqtadar,Kenji Hirayama,Pham Thi Le Hoa,Nguyen Tien Huy

Archives of microbiology 206:41 PubMed38147133

2023

A robust CRISPR interference gene repression system in Vibrio parahaemolyticus.

Applications

Unspecified application

Species

Unspecified reactive species

Taoyuan Jiang,Yuhuan Li,Wencong Hong,Mingyu Lin

EBioMedicine 85:104311 PubMed36374770

2022

Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Andras T Meszaros,Julia Hofmann,Madita L Buch,Benno Cardini,Theresia Dunzendorfer-Matt,Florian Nardin,Michael J Blumer,Margot Fodor,Martin Hermann,Bettina Zelger,Giorgi Otarashvili,Melanie Schartner,Annemarie Weissenbacher,Rupert Oberhuber,Thomas Resch,Jakob Troppmair,Dietmar Fner,Heinz Zoller,Herbert Tilg,Erich Gnaiger,Theresa Hautz,Stefan Schneeberger

Cells 11: PubMed36359822

2022

Glutamate Prevents Altered Mitochondrial Function Following Recurrent Low Glucose in Hypothalamic but Not Cortical Primary Rat Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Paul G Weightman Potter,Kate L J Ellacott,Andrew D Randall,Craig Beall

Biomolecules & therapeutics 30:409-417 PubMed35611585

2022

Sinapic Acid Ameliorates REV-ERB α Modulated Mitochondrial Fission against MPTP-Induced Parkinson's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Bin Lee,Hyun Ok Yang

Cell reports 39:110660 PubMed35417703

2022

SENP1-Sirt3 signaling promotes α-ketoglutarate production during M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Gaolei Hu,Jianli He,Tianshi Wang,Yong Zuo,Ying Cao,Quan Zheng,Jun Tu,Jiao Ma,Rong Cai,Yalan Chen,Qiuju Fan,Baijun Dong,Hongsheng Tan,Qi Wang,Wei Xue,Jinke Cheng

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:1410-1424 PubMed35296173

2022

Aging related impairment of brain microvascular bioenergetics involves oxidative phosphorylation and glycolytic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Siva Svp Sakamuri,Venkata N Sure,Lahari Kolli,Wesley R Evans,Jared A Sperling,Gregory J Bix,Xiaoying Wang,Dmitriy N Atochin,Walter L Murfee,Ricardo Mostany,Prasad Vg Katakam

Life (Basel, Switzerland) 12: PubMed35054496

2022

Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin.

Applications

Unspecified application

Species

Unspecified reactive species

Annie John,Layla Amiri,Jasmin Shafarin,Saeed Tariq,Ernest Adeghate,Frank Christopher Howarth,Haider Raza
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com