Glucose-6-Phosphate Assay试剂盒(Colorimetric)
Glucose-6-Phosphate Assay Kit (Colorimetric)
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(19 Publications)
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- FuncS
PubMed
Functional Studies - Glucose-6-Phosphate Assay Kit (Colorimetric) (AB83426)
G6P (A) levels are elevated in GAA−/− mice compared to wild type (C57/Bl6) and reduced by rhGAA treatment.
GAA−/− mice were dosed with rhGAA. G6P was quantified in heart and triceps homogenates from wild type (C57Bl/6) and GAA−/− mice using ab83426. Values are means ± SEM. Data was analyzed by one-way ANOVA followed by Newman-Keuls comparing groups. ***P<0.001.
Image from Taylor KM et al., PLoS One. 2013;8(2):e56181. Fig 6(A).; doi: 10.1371/journal.pone.0056181. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- FuncS
Lab
Functional Studies - Glucose-6-Phosphate Assay Kit (Colorimetric) (AB83426)
Colorimetric standard curve : mean of duplicates (+/- SD) with background reads subtracted.
产品详情
Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426) is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized into an intermediate product which in turn converts the probe into an intensely colored product with an absorbance at OD=450 nm. This product can detect G6P in the range of 1-30 nmoles with a detection sensitivity ~10 uM G6P.
For higher sensitivity, we recommend fluorometric assay Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) ab107923.
Other Notes
This product was previously called K657 Biovision Glucose-6-Phosphate Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is transformed by G6PDH to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
REACH authorisation
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Glucose 6-phosphate acts as a substrate for several biochemical processes within the cell. It is an important component in glycogen synthesis and the pentose phosphate pathway providing important intermediates for nucleic acid and lipid synthesis. Although it is not part of a complex in itself glucose 6-phosphate interacts with various enzymes to activate these pathways reflecting its functional significance.
Pathways
Glucose 6-phosphate is a critical connector in glycolysis and gluconeogenesis. In glycolysis the molecule continues through a series of enzymatic reactions to generate ATP which cells use for energy. Meanwhile in gluconeogenesis glucose 6-phosphate can be converted back to glucose. G6P acts in coordination with enzymes such as glucose-6-phosphate dehydrogenase in the pentose phosphate pathway highlighting its multifunctional nature and its interaction with other proteins like phosphoglucomutase.
文献 (19)
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