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Fumarase Specific Activity Assay试剂盒(ab110043)

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Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
  • Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader

概述

  • 产品名称

    Fumarase Specific Activity Assay试剂盒

  • 检测方法

    Colorimetric

  • 检测类型

    Enzyme activity

  • 种属反应性

    与反应: Rat, Cow, Human
    不与反应: Mouse

  • 产品概述

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • 说明

    ab110043 (MS780) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate fumarase (FH), which removes all other enzymes of the Krebs cycle and NAD+/NADH utilizing enzymes. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated FH is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • 平台

    Microplate reader

性能

图片

  • Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
    Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
    Example data obtained using the Fumarase Specific Activity Assay Kit (ab110043).
  • Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
    Sandwich ELISA - Fumarase Specific Activity Assay Kit (ab110043)
    Principle of a Sandwich ELISA.

文献 (3)

发表研究结果有使用 ab110043？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110043 被引用在 3 文献中.

  • Chatre L  et al. Increased NOS coupling by the metabolite tetrahydrobiopterin (BH4) reduces preeclampsia/IUGR consequences. Redox Biol 55:102406 (2022). PubMed: 35964341
  • Wang Z  et al. A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX. PLoS Pathog 15:e1007791 (2019). PubMed: 31545842
  • Perrier-Trudova V  et al. Fumarate Hydratase-deficient Cell Line NCCFH1 as a New In Vitro Model of Hereditary Papillary Renal Cell Carcinoma Type 2. Anticancer Res 35:6639-53 (2015). PubMed: 26637880

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Question

In particular, there are discrepancies in the units used throughout the instructions. The range of lysate dilutions to use for various cell lines is in ug/mL. However, in the data analysis sectionm, units of mg/mL are used. What is the correct range of lysate concentrations I should be using? For example, 250ug/mL OR 250mg/mL? I am guessing ug/mL but this is not clear from the instructions. -I do not see much activity for my samples. What is the typical change in absorbance values observed for a sample that has FH activity?

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Answer

Thank you for contacting us and for bringing this error to our attention.

The units should all me in ug, not mg.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I had a customer with a product issue.They hadn’t realized that it’s supposed to react with mouse and wants to use it with human but is concerned about it working (I see now from the datasheet that it does react with human but not with mouse, so I suspect she had it backwards).

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Answer

Thank you for contacting us.

I can confirm that ab110043 Fumarase Specific Activity Assay Kit does worki with Rat, Cow and Human samples. This product will not work with mouse.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Has the fumarase activity kit ab110043 been tested for reactivity with mouse samples?

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Answer

Thank you for contacting us.

The antibody used in this kit detects only human, rat and cow FH. It does not detect it in mouse samples. Therefore, we do not expect to see the activity from mouse samples in this immunocapture based activity assay.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Has the fumarase activity kit ab110043 been tested for reactivity with mouse samples?
Is the microplate for the ATP synthase activity assay ab109714 a standard microplate, or 12 strips of 8 wells each in a frame?

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Answer

I am still waiting for mouse reactivity data for the fumarase assay, ab110043. I did confirm, however, that the ATP synthase assay, ab109714, does come with 12 strips of 8 wells each, and not a standard solid microplate.

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Question

I am interested in learning more about Abreviews to earn Abpoints.

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Answer

Thank you very much for your interest in our products.

I just sent you Proforma Invoice (quotation) #45550 for the 4 kits. This quote will be good until Sept 30th when the discount expires. When you're ready to place the order, just call us and mention this number. If you need more reagents for your studies, you can use the discount code 4FOR3-TB3ME through Sept 30th to get the 4 for the price of 3 promotion on all products.

We would be very grateful if you could submit an Abreview aboutthese productsvia the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. You would have to do this manually, by working with our Scientific Support team through mailto:us.technical@abcam.com.

To find out more about our Abreview system, please see the following webpage: https://www.abcam.com/abreviews

And Abpoints: https://www.abcam.com/index.html?pageconfig=loyalty_rewards_list

We hope to receive your Abreviews. Thank you for your time and effort.

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