Fructose-1,6-Bisphosphate Assay试剂盒(Fluorometic) (ab284537)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Cell Lysate, Tissue, Tissue Lysate
概述
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产品名称
Fructose-1,6-Bisphosphate Assay试剂盒(Fluorometic) -
检测方法
Fluorescent -
样品类型
Tissue, Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
实验步骤
Multiple steps standard assay -
产品概述
This Fructose-1,6-Bisphosphate Assay Kit (ab284537) (previously called PicoProbe Fructose-1,6-Bisphosphate Assay Kit (Fluorometric) K2036) provides a quick and easy way to measure Fructose-1,6-Bisphosphate (F1,6BP) in a variety of sample types. In this Assay, F1,6BP is hydrolyzed by Fructose-1,6-Bisphosphatase (FBP) into inorganic phosphate and F6P. F6P then through several intermediate reactions reacts with the PicoProbe to generate a stable fluorophore measured at Ex/Em = 535/587 nm.
The assay is simple, easy to perform, sensitive and can detect F1,6BP less than 0.5 µM in a variety of samples.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K2036 PicoProbe™ Fructose-1,6-Bisphosphate Assay Kit (F). K2036-100 is the same size as the 100 test size of ab284537.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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经测试应用
适用于: Functional Studiesmore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer V 1 x 25ml Converter Enzyme X 1 vial Developer IX 1 vial Development Enzyme Mix IX 1 vial F1,6BP Enzyme 1 vial F1,6BP Standard 4 vials PicoProbe I 1 x 400µl
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab284537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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PicoProbe™ Fructose-1,6-Bisphosphate Assay Kit (Fluorometic)
Fructose-1,6-Bisphosphate Standard Curve generated using this kit.
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PicoProbe™ Fructose-1,6-Bisphosphate Assay Kit (Fluorometic)Measurement of Fructose-1,6-Bisphosphate in mouse liver lysate (50 µg protein) and germinated barley seed lysate (G7) (10 µg protein). Assays were performed following the protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab284537 尚未被引用在任何文献中。