Fructose-1,6-Bisphosphatase Activity Assay试剂盒(Colorimetric) (ab273329)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
概述
-
产品名称
Fructose-1,6-Bisphosphatase Activity Assay试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Enzyme activity (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329) provides a quick, reliable test that allows the measurement of Fructose-1,6-Bisphosphatase (FBP) enzymatic activity in various samples. In this assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm.
The assay is simple, sensitive and can detect lower than 100 µU of FBP in variety of samples.
-
说明
This product is manufactured by BioVision, an Abcam company and was previously called K590 Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric). K590-100 is the same size as the 100 test size of ab273329.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer V 1 x 25ml Converter Enzyme X 1 vial Developer IX 1 vial F6P Standard 1 vial FBP Positive Control 1 vial FBP Substrate 1 vial NADPH Substrate Mix 1 vial -
研究领域
-
细胞定位
FBP2: Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration.
图片
-
Example data
Example data.
F6P standard curve.
-
Example dataExample data.
Kinetic measurement of Fructose-1,6-Biosphophatase activity from various samples.
-
Example dataExample data.
Specific FBP Activity was calculated in lysates prepared from rat liver (3.2µg protein), rat kidney (8µg protein) and HeLa lysate (4µg protein).
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab273329 被引用在 2 文献中.
- Gu L et al. Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness. Cell Metab 35:1009-1021.e9 (2023). PubMed: 37084733
- Pack M et al. Protein Kinase CK2 Contributes to Glucose Homeostasis by Targeting Fructose-1,6-Bisphosphatase 1. Int J Mol Sci 24:N/A (2022). PubMed: 36613872