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Formate Assay试剂盒(ab111748)

Submit a review Q&A (2)References (3)
Functional Studies - Formate Assay Kit (ab111748)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 1 hr
    • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine

    概述

    • 产品名称

      Formate Assay试剂盒

    • 检测方法

      Colorimetric

    • 样品类型

      Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate

    • 检测类型

      Quantitative

    • 检测时间

      1h 00m

    • 产品概述

      In Abcam's Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.


      Visit our FAQs page for tips and troubleshooting.

    • 说明

      This product is manufactured by BioVision, an Abcam company and was previously called K653 Formate Colorimetric Assay Kit. K653-100 is the same size as the 100 test size of ab111748.

      Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.

      The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

    • 平台

      Microplate reader

    性能

    图片

    • Functional Studies - Formate Assay Kit (ab111748)
      Functional Studies - Formate Assay Kit (ab111748)
      Example of standard curve obtained using ab111748

    文献 (3)

    发表研究结果有使用 ab111748？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab111748 被引用在 3 文献中.

    • Wang Y  et al. Systematic evaluation of urinary formic acid as a new potential biomarker for Alzheimer's disease. Front Aging Neurosci 14:1046066 (2022). PubMed: 36533170
    • Li G  et al. p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34244426
    • Oizel K  et al. Formate induces a metabolic switch in nucleotide and energy metabolism. Cell Death Dis 11:310 (2020). PubMed: 32366892

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    I was wondering what the assay buffer of the kit contains.

    We are concerned with the activity of native formate dehydrogenase in the homogenous samples.

    Is there any way to inactivate those enzymes without disturbing the endogenous formate? We wanted to try boiling the homogenous samples, but we were not sure if we would disrupt some component of the buffer or not.

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    Answer

    Thank you for contacting Abcam

    The recipe for the Formate assay buffer is proprietary, but it does contain some NP-40 (< 0.5 %).

    Please let me know if you have any other questions.

    Read More

    Question

    To Abcam technical support,

    I am using the kit to measure formate level in cyanobacteria. Cyanobacteria is gram-negative, but their peptidoglycan layer is thicker, and they also have cell walls.

    My question is, what homogenization protocol would you recommend to break the cells? We normally use protease K to lyse cells, but I am afraid it may interfere with the assay kit's enzymatic activity.

    I hope to hear back from you soon,

    Read More
    Answer

    Thank you for your enquiry.

    I agree that perhaps a mechanical extraction method would be best for this assay. Here is a link to a publication that discusses a variety of different methods to produce cell extracts from cyanobacteria.

    http://www.ceric.net/wonmun3/ksee/2009_14_4_EER_417.pdf

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

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