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AB272524

Formaldehyde Assay试剂盒

Formaldehyde Assay Kit

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(1 Publication)

Formaldehyde Assay Kit (ab272524) is a mix-and-read assay with a single 30-min incubation at room temperature. Readout on any fluorometric (Ex/Em 370/470 nm) plate reader.
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Functional Studies - Formaldehyde Assay Kit (AB272524)
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Supplier Data

Functional Studies - Formaldehyde Assay Kit (AB272524)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

关键信息

检测方法

Fluorescent

样品类型

Urine, Plasma, Serum, Other biological fluids, Cell Lysate

检测类型

Quantitative

灵敏度

= 1.5 µM

检测平台

Microplate

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产品详情

Formaldehyde Assay Kit (ab272524) provides a convenient fluorimetric means to measure formaldehyde in biological samples.

In the Formaldehyde assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia. The resulting fluorescent product is then quantified fluorimetrically (Ex/Em 370/470nm)

Formaldehyde assay protocol summary
Prepare all reagents and samples as instructed.
- Add samples and standards to wells
- Add working reagent
- Incubate for 30 minutes at RT protected from light.
- analyse on plate reader

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规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab272524-100Test", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"Reagent B", "number":"AB272524-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"TCA (10%)", "number":"AB272524-CMP04", "productcode":"" }, { "size":"2 x 1.5 mL", "name":"Neutralizer", "number":"AB272524-CMP05", "productcode":"" }, { "size":"1 x 5 mL", "name":"Reagent A", "number":"AB272524-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Standard", "number":"AB272524-CMP03", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Formaldehyde also known as methanal is the simplest form of aldehyde with a molecular mass of 30.03 g/mol. It naturally occurs as a gas and dissolves in water to form formalin. Inside living organisms formaldehyde is endogenously produced and can be expressed in various tissues including liver and kidneys. Formaldehyde influences several cellular processes by modifying proteins and nucleic acids through the formation of cross-links affecting their function and stability.
Biological function summary

Formaldehyde plays a significant role in cell metabolism and gene regulation. It participates in the one-carbon cycle as part of the detoxification system. The enzyme formaldehyde dehydrogenase aids in its conversion to formate which cells can utilize for nucleotide synthesis and methylation reactions. This association makes formaldehyde a part of the folate-mediated one-carbon metabolic pathway essential for DNA synthesis and repair.

Pathways

Formaldehyde is involved in metabolic processes like the methionine and serine cycles which are critical for amino acid interconversion and methyl group donation. It shares significant interaction with proteins such as S-adenosylmethionine (SAM) known for its role as a major methyl donor in various biological reactions. These interactions highlight formaldehyde's importance in maintaining cellular homeostasis and epigenetic regulation.

Formaldehyde exposure and metabolism have been linked to certain types of cancers and neurodegenerative diseases. Its accumulation can lead to DNA and protein damage contributing to carcinogenesis and diseases like Alzheimer's where tangles and plaques play a major role. Proteins such as amyloid-beta which are involved in these disorders can be affected by formaldehyde-induced modifications further implicating its influence in disease pathogenesis.
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产品实验方案

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靶点信息

See full target information Formaldehyde
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 12: PubMed37626940

2023

High-Throughput Assay of Cytochrome P450-Dependent Drug Demethylation Reactions and Its Use to Re-Evaluate the Pathways of Ketamine Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nadezhda Y Davydova,David A Hutner,Kari A Gaither,Dilip Kumar Singh,Bhagwat Prasad,Dmitri R Davydov
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