Factor XII / XIIa Assay试剂盒
Factor XII / XIIa Assay Kit
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Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).
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Coagulation factor XII, Hageman factor, HAF, F12
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Lab
Functional Studies - Factor XII / XIIa Assay Kit (AB241041)
Factor XII is converted to it’s active form (XIIa) by the Coagulation Activator. Factor XIIa cleaves Prekallikrein into active Kallikrein which in turn cleaves the substrate releasing a colorimetric product. In the presence of the inhibitor, Factor XIIa is inactivated (XIIi) by the inhibitor and cannot activate Prekallikrein.
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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)
Kinetic progressive curves for different amounts of FXIIa Enzyme
- FuncS
Unknown
Functional Studies - Factor XII / XIIa Assay Kit (AB241041)
Standard curve for pNA.
- FuncS
Unknown
Functional Studies - Factor XII / XIIa Assay Kit (AB241041)
Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.
- FuncS
Unknown
Functional Studies - Factor XII / XIIa Assay Kit (AB241041)
PK activity. Standard curve for pNA (n = 3) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.
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The Factor XIIa Assay Kit (ab241041) utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples. This kit also measures Factor XII. In the sample preparation, the FXII is converted to FXIIa by the FXII activator.
This product is manufactured by BioVision, an Abcam company and was previously called K994 Factor XIIa Activity Assay Kit (Colorimetric). K994-100 is the same size as the 100 test size of ab241041.
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