Ethanol Assay试剂盒(ab272531)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Sample type: Cell culture media, Food samples, Saliva, Urine
概述
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产品名称
Ethanol Assay试剂盒
参阅全部 Ethanol 试剂盒 -
检测方法
Colorimetric -
样品类型
Saliva, Urine, Cell culture media, Food samples -
检测类型
Quantitative -
范围
0.04 % - 2 % -
产品概述
Ethanol Assay Kit (ab272531) is a simple, direct and automation-ready procedure for measuring ethanol concentration. This assay kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Sensitive and accurate: Detection range 0.04 – 2% alcohol in 96-well plate assay.
Convenient and high-throughput: The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a highthroughput 96-well plate assay for thousands of samples per day.
Versatility: Assays can be executed in 96-well plate or cuvet.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Functional Studiesmore details
性能
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存放说明
Please refer to protocols. -
组件 500 tests 500 tests Reagent A 1 x 50ml 1 x 50ml Reagent B 1 x 50ml 1 x 50ml Standard 1 x 1.5ml 1 x 1.5ml TCA (10%) 1 x 50ml 1 x 50ml -
研究领域
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相关性
Alcohol (ethanol C2H5OH) is a clear colorless liquid rapidly absorbed from the gastrointestinal tract and distributed through the body. Ethanol has a depressive effect on the central nervous system and because of its psychoactive effects, it is considered a drug. Ethanol is metabolized by the body as an enerfy-providing carbohydrate nutrient, as it metabolized into acetyl CoA, an intermediate common with glucose than can be used for energy in the citric acid cycle (TCA) or for biosynthesis. Ethanol within the human body is converted into acetaldehyde by alcohol dehydrogenase and then into acetic acid by acetaldehyde dehydriogenase. Acetaldehyde, the product of the first step of the alcohol breakdown, is linked to most of the toxic clinical effects of alcohol. -
别名
- Ethyl alcohol
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272531于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Ethanol Assay Kit standard curve in 96-well plate
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab272531 被引用在 2 文献中.
- Altounian M et al. Neuronal miR-17-5p contributes to interhemispheric cortical connectivity defects induced by prenatal alcohol exposure. Cell Rep 42:113020 (2023). PubMed: 37610874
- Böhm J et al. Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria. Front Microbiol 14:1238737 (2023). PubMed: 37649635