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AB241024

Enolase Assay试剂盒

Enolase Assay Kit

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(4 Publications)

In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product.

查看别名

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE

3 Images
Functional Studies - Enolase Assay Kit (AB241024)
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Functional Studies - Enolase Assay Kit (AB241024)

Enolase Activity in rat muscle lysate (1 μg), HeLa lysate (5 μg) and positive control. Assays were performed following the kit protocol.

Functional Studies - Enolase Assay Kit (AB241024)
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Functional Studies - Enolase Assay Kit (AB241024)

H2O2 Standard Curve for Fluorometric Assay.

H2O2 Standard Curve for Fluorometric Assay.

Functional Studies - Enolase Assay Kit (AB241024)
  • FuncS

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Functional Studies - Enolase Assay Kit (AB241024)

H2O2 Standard Curve for Colorimetric Assay.

H2O2 Standard Curve for Colorimetric Assay.

关键信息

检测方法

Colorimetric/Fluorometric

样品类型

Tissue, Suspension cells, Cell culture supernatant, Adherent cells

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

In the Enolase Assay Kit (ab241024) enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with the probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm).

This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.

This product is manufactured by BioVision, an Abcam company and was previously called K691 Enolase Activity Colorimetric/Fluorometric Assay Kit. K691-100 is the same size as the 100 test size of ab241024.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241024-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Enolase Positive Control", "number":"AB241024-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enolase Converter Mix", "number":"AB241024-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"2-Phosphoglycerate", "number":"AB241024-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB241024-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"H2O2 Standard", "number":"AB241024-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB241024-CMP07", "productcode":"" }, { "size":"1 x 1 Each", "name":"Developer Mix Q", "number":"AB241024-CMP04", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.
Biological function summary

The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.

Pathways

ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.

ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.
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产品实验方案

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靶点信息

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
See full target information ENO1
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:10006-10019 PubMed40180688

2025

α-Enolase and γ-Enolase Expression in Enriched S- and N-Type SH-SY5Y Cells: Regulatory Role of Cathepsin X.

Applications

Unspecified application

Species

Unspecified reactive species

Selena Horvat,Nace Zidar,Janko Kos,Anja Pišlar

Biomedicines 11: PubMed37189694

2023

Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Zoran Minic,Yingxi Li,Nico Hüttmann,Gurcharan K Uppal,Rochelle D'Mello,Maxim V Berezovski

Evidence-based complementary and alternative medicine : eCAM 2022:9302403 PubMed35502178

2022

Salvianolic Acid B Suppresses Non-Small-Cell Lung Cancer Metastasis through PKM2-Independent Metabolic Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhang,Jianming Tang,Yu Cao,Tianhu Wang

Cancer cell 37:599-617.e7 PubMed32243837

2020

KMT2D Deficiency Impairs Super-Enhancers to Confer a Glycolytic Vulnerability in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hunain Alam,Ming Tang,Mayinuer Maitituoheti,Shilpa S Dhar,Manish Kumar,Chae Young Han,Chandrashekar R Ambati,Samir B Amin,Bingnan Gu,Tsai-Yu Chen,Yu-Hsi Lin,Jichao Chen,Florian L Muller,Nagireddy Putluri,Elsa R Flores,Francesco J DeMayo,Laura Baseler,Kunal Rai,Min Gyu Lee
View all publications
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