ECL底物试剂盒(Very High Sensitivity) (ab133408)
概述
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产品名称
ECL底物试剂盒(Very High Sensitivity)
参阅全部 ECL Substrate 试剂盒 -
产品概述
Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging. It is designed for the detection of proteins from 4.6pg to 4.7ng of protein per band.
ECL Substrate Kit ab133408 produces a strong, extremely long-lived signal, which, combined with very low background levels, allows for long exposure times enabling the detection of low-abundance proteins. Additionally, the signal from the ECL substrate is linear with respect to protein amount over a broad range of concentrations, displaying no substrate depletion at high protein loads, allowing the user to take full advantage of the linear range of the CCD detection method.
The ECL substrate is also compatible with X-ray film detection, though the limited dynamic range of film will make resulting data less quantitative.
Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
- this kit (Very High Sensitivity ECL Substrate Kit ab133408) to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Max Detect Kit (4.6pg-4.7ng).
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说明
Use:
- 100ml kit for 1000cm2 membrane
- 200ml kit for 2000cm2 membraneThe primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.
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经测试应用
适用于: WBmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 200 ml 100 ml 20 ml Luminol/enhancer solution 1 x 100ml 1 x 50ml 1 x 10ml Peroxide Chemiluminescent Detection Reagent 1 x 100ml 1 x 50ml 1 x 10ml -
研究领域
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133408于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
图片
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Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) at 1/50000 dilution and with a rabbit secondary (ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Max Detect.
Exposure time: 10 seconds
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Each lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) at 1/25000 dilution and with a rabbit secondary (ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Max Detect.
Exposure time: 10 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133408 被引用在 2 文献中.
- Zhou Z et al. Rebalancing TGF-β/PGE2 breaks RT-induced immunosuppressive barriers by enhancing tumor-infiltrated dendritic cell homing. Int J Biol Sci 20:367-386 (2024). PubMed: 38164187
- Wege S & Poirier Y Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export. FEBS Lett 588:482-9 (2014). PubMed: 24374333