ECL底物试剂盒(High Sensitivity) (ab133406)
概述
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产品名称
ECL底物试剂盒(High Sensitivity)
参阅全部 ECL Substrate 试剂盒 -
产品概述
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.
Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Detect Kit (23pg-187ng).
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说明
Use:
- 200ml kit for 2000cm2 membrane
- 500ml kit for 5000cm2 membraneThe primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.
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经测试应用
适用于: WBmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 200 ml 20 ml 500 ml Luminol/Enhancer Solution 1 x 100ml 1 x 10ml 1 x 250ml Peroxide Chemiluminescent Detection Reagent 1 x 100ml 1 x 10ml 1 x 250ml -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133406于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. |
图片
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Western blot comparison 1/25000 anti-Transferrin primary antibody, 1/50000 secondary antibody, 20 second exposure
Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.
Secondary
HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.
Exposure time: 20 seconds
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Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 20 second exposureEach lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) at 1/25000 dilution and with a rabbit secondary (ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Detect.
Exposure time: 20 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (58)
ab133406 被引用在 58 文献中.
- Alhawamdeh M et al. Effect of IFN‑γ encapsulated liposomes on major signal transduction pathways in the lymphocytes of patients with lung cancer. Oncol Lett 27:8 (2024). PubMed: 38028180
- Ling M et al. IL1A regulates the inflammation in gout through the Toll-like receptors pathway. Int J Med Sci 21:188-199 (2024). PubMed: 38164346
- Eymery MC et al. Linking medicinal cannabis to autotaxin-lysophosphatidic acid signaling. Life Sci Alliance 6:N/A (2023). PubMed: 36623871
- Nie XJ et al. GATA3 ameliorates melanocyte injuries in vitiligo through SIRT3-mediated HMGB1 deacetylation. J Dermatol 50:472-484 (2023). PubMed: 36412048
- Zhao H et al. SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosis. Open Med (Wars) 18:20220620 (2023). PubMed: 36820068