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Epigenetics and Nuclear Signaling DNA methylation DNMTs

DNMT1 Assay试剂盒(ab113469)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (8)

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Functional Studies - DNMT1 Assay Kit (ab113469)

    Key features and details

    • Assay type: Quantitative
    • Platform: Microplate reader
    • Assay time: 3 hr
    • Sample type: Cell Lysate, Tissue Extracts

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    概述

    • 产品名称

      DNMT1 Assay试剂盒
    • 样品类型

      Tissue Extracts, Cell Lysate
    • 检测类型

      Quantitative
    • 检测时间

      3h 00m
    • 种属反应性

      与反应: Mouse, Human
      预测可用于: Mammals
    • 产品概述

      DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.

       DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.

    • 平台

      Microplate reader

    性能

    • 存放说明

      Please refer to protocols.
    • 组件 96 tests 48 tests
      10X Wash Buffer 1 x 28ml 1 x 14ml
      8-Well Assay Strips (with Frame) 12 units 6 units
      Capture Antibody, 500 µg/mL 1 x 16µl 1 x 8µl
      Assay Buffer 1 x 10ml 1 x 5ml
      Blocking Buffer 1 x 20ml 1 x 10ml
      Detection Antibody, 200 µg/mL 1 x 20µl 1 x 10µl
      Developing Solution 1 x 12ml 1 x 6ml
      DNMT1 Standard, 20 µg/mL 1 x 40µl 1 x 20µl
      Stop Solution 1 x 11ml 1 x 6ml
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • DNA methylation
      • DNMTs
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • DNA modification and methylation

    图片

    • Functional Studies - DNMT1 Assay Kit (ab113469)
      Functional Studies - DNMT1 Assay Kit (ab113469)

      Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and DNMT1 activity was measured using ab113469. DNMT3A and DNMT3B activities were measured using ab113470 DNMT3A Assay Kit and ab113471 EpiSeeker DNMT3B Assay Kit.

    实验方案

    • Protocol Booklet

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab113469?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab113469 被引用在 8 文献中.

    • Chen M  et al. The activation of mTOR signalling modulates DNA methylation by enhancing DNMT1 translation in hepatocellular carcinoma. J Transl Med 21:276 (2023). PubMed: 37088830
    • Diao W  et al. Expression and Clinical Significance of HER2 Gene and DNMT1 in Non-Small-Cell Lung Cancer. Dis Markers 2022:8426384 (2022). PubMed: 35996715
    • Zhu H  et al. Sennoside A prevents liver fibrosis by binding DNMT1 and suppressing DNMT1-mediated PTEN hypermethylation in HSC activation and proliferation. FASEB J 34:14558-14571 (2020). PubMed: 32946656
    • Xia X  et al. Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer. Microbiome 8:108 (2020). PubMed: 32678024
    • Ferreira LL  et al. Single nanomolar doxorubicin exposure triggers compensatory mitochondrial responses in H9c2 cardiomyoblasts. Food Chem Toxicol 124:450-461 (2018). PubMed: 30557669
    • Ramaiyan B & Talahalli RR Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats. Lipids 53:581-588 (2018). PubMed: 30203512
    • Ma SC  et al. Homocysteine-induced oxidative stress through TLR4/NF-?B/DNMT1-mediated LOX-1 DNA methylation in endothelial cells. Mol Med Rep 16:9181-9188 (2017). PubMed: 29039510
    • Yoon JH  et al. NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells. Sci Rep 7:2807 (2017). PubMed: 28584243

    客户评价及客户问答

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    DNMT1 quantification in H9c2 nuclear fractions

    Good Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    We used the DNMT1 assay kit (ab113469) to quantify DNMT1 in nuclear fractions of H9c2 cardiomyoblasts. The objective was to compare the amount of DNMT1 in doxorubicin-treated cells with the controls. The cells were cultivated in DMEM medium and drugged with 0,025 µM of doxorubicin for 24 hours. To have a considerable signal we needed to use 6 micrograms of nuclear protein enriched fraction. The kit was easy to use and the results were consistent.
    As negative aspects of this kit we point out the fact that the assay instructions are incomplete and some reagents are not even mentioned in the procedure.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

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    提交于 Aug 07 2015

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