DNMT1 Assay试剂盒(ab113469)
Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell Lysate, Tissue Extracts
概述
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产品名称
DNMT1 Assay试剂盒 -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Quantitative -
检测时间
3h 00m -
种属反应性
与反应: Mouse, Human
预测可用于: Mammals
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产品概述
DNMT1 (DNA (cytosine-5)-methyltransferase 1) belongs to the DNMT family, responsible for methylation of CpG islands in the genome. DNMT1 preferably methylates cytosines located in hemimethylated DNA and it associates with DNA replication sites in S-phase.
DNMT1 Assay Kit (ab113469) allows the user to quickly and consistently measure the amount of DNMT1. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT1 assay experiment without the need for electrophoresis and transfer process. ab113469 is suitable for measuring DNMT amounts quantitatively from fresh tissue and cultured cells of human and mouse.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 96 tests 48 tests 10X Wash Buffer 1 x 28ml 1 x 14ml 8-Well Assay Strips (with Frame) 12 units 6 units Capture Antibody, 500 µg/mL 1 x 16µl 1 x 8µl Assay Buffer 1 x 10ml 1 x 5ml Blocking Buffer 1 x 20ml 1 x 10ml Detection Antibody, 200 µg/mL 1 x 20µl 1 x 10µl Developing Solution 1 x 12ml 1 x 6ml DNMT1 Standard, 20 µg/mL 1 x 40µl 1 x 20µl Stop Solution 1 x 11ml 1 x 6ml -
研究领域
图片
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Functional Studies - DNMT1 Assay Kit (ab113469)
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab113469 被引用在 8 文献中.
- Chen M et al. The activation of mTOR signalling modulates DNA methylation by enhancing DNMT1 translation in hepatocellular carcinoma. J Transl Med 21:276 (2023). PubMed: 37088830
- Diao W et al. Expression and Clinical Significance of HER2 Gene and DNMT1 in Non-Small-Cell Lung Cancer. Dis Markers 2022:8426384 (2022). PubMed: 35996715
- Zhu H et al. Sennoside A prevents liver fibrosis by binding DNMT1 and suppressing DNMT1-mediated PTEN hypermethylation in HSC activation and proliferation. FASEB J 34:14558-14571 (2020). PubMed: 32946656
- Xia X et al. Bacteria pathogens drive host colonic epithelial cell promoter hypermethylation of tumor suppressor genes in colorectal cancer. Microbiome 8:108 (2020). PubMed: 32678024
- Ferreira LL et al. Single nanomolar doxorubicin exposure triggers compensatory mitochondrial responses in H9c2 cardiomyoblasts. Food Chem Toxicol 124:450-461 (2018). PubMed: 30557669
- Ramaiyan B & Talahalli RR Dietary Unsaturated Fatty Acids Modulate Maternal Dyslipidemia-Induced DNA Methylation and Histone Acetylation in Placenta and Fetal Liver in Rats. Lipids 53:581-588 (2018). PubMed: 30203512
- Ma SC et al. Homocysteine-induced oxidative stress through TLR4/NF-?B/DNMT1-mediated LOX-1 DNA methylation in endothelial cells. Mol Med Rep 16:9181-9188 (2017). PubMed: 29039510
- Yoon JH et al. NKX6.3 Regulates Reactive Oxygen Species Production by Suppressing NF-kB and DNMT1 Activities in Gastric Epithelial Cells. Sci Rep 7:2807 (2017). PubMed: 28584243