Dihydroxyacetone Phosphate (DHAP) Assay试剂盒(Fluorometric) (ab197003)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 0.5 µM
概述
-
产品名称
Dihydroxyacetone Phosphate (DHAP) Assay试剂盒(Fluorometric) -
检测方法
Fluorescent -
样品类型
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
检测类型
Quantitative -
灵敏度
> 0.5 µM -
种属反应性
与反应: Mammals, Other species -
产品概述
Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003) is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, triose phosphate isomerase (TPI) converts dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP), which subsequently undergoes a series of reaction and reduces the probe to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of dihydroxyacetone Phosphate.
Detection limit: 0.5 µM DHAP.
-
说明
This product is manufactured by BioVision, an Abcam company and was previously called K673 PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit. K673-100 is the same size as the 100 test size of ab197003.
Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, fructose-1,6- diphosphate is converted to dydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular pool for triose phosphate. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI).
In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
-
平台
Microplate reader
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer II 1 x 25ml Developer Solution X 1 vial DHAP Enzyme Mix 1 vial DHAP Standard 1 vial PicoProbe I 1 x 400µl
图片
-
Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)
Typical DHAP Standard Curve obtained following assay protocol.
-
Functional Studies - Dihydroxyacetone Phosphate (DHAP) Assay Kit (Fluorometric) (ab197003)Measurement of DHAP level in a varity of samples: Jurkat (250 µg),and MCF-7 (150 µg) cell lysate, and in rat liver (50 µg) and rat muscle (150 µg) tissue lysate.
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab197003 被引用在 3 文献中.
- Haythorne E et al. Altered glycolysis triggers impaired mitochondrial metabolism and mTORC1 activation in diabetic β-cells. Nat Commun 13:6754 (2022). PubMed: 36376280
- Parvaiz N et al. Discovery of beta-lactamase CMY-10 inhibitors for combination therapy against multi-drug resistant Enterobacteriaceae. PLoS One 16:e0244967 (2021). PubMed: 33449932
- Jiang H et al. The Mitochondria-Targeted Metabolic Tubular Injury in Diabetic Kidney Disease. Cell Physiol Biochem 52:156-171 (2019). PubMed: 30816665