Cytochrome c Apoptosis ICC抗体试剂盒(ab110417)
Key features and details
- Assay type: Direct
- Detection method: Fluorescent
- Sample type: Adherent cells
概述
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产品名称
Cytochrome c Apoptosis ICC抗体试剂盒
参阅全部 ApoTrack™ Cytochrome c Apoptosis 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells -
检测类型
Direct -
种属反应性
与反应: Mouse, Rat, Human -
产品概述
Apoptotic cells which have released mitochondrial cytochrome c into the cytosol can be differentiated from non apoptotic cells which still retain cytochrome c in their mitochondria by fluorescence microscopy.
ab110417 (MSA07) contains a cytochrome c monoclonal antibody (50 µg) and an isotype-specific secondary antibody (50 µg) which has a fluorescein fluorophore conjugated to it. The kit also contains an ATP synthase V subunit alpha monoclonal antibody (50 µg) and the appropriate isotype-specific secondary antibody (50 µg) which has a Texas Red fluorophore conjugated to it.
ATP synthase is an inner mitochondrial membrane protein and serves as a mitochondrial marker since it is not released into the cytoplasm during apoptosis, unlike cytochrome c. Thus one is able to observe cytochrome c and a mitochondrial marker (ATP synthase subunit alpha) simultaneously by fluorescence microscopy using ab110417. Buffers and blocking solution for immunocytochemical analysis are also included in the kit.
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说明
This product was previously called ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit.
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: ICC/IFmore details
性能
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存放说明
Please refer to protocols. -
组件 标识符 200 µg 100% normal goat serum 1 x 20ml 10X Buffer (10X PBS, pH 7.4) 1 x 100ml Anti-Complex Va monoclonal antibody clone 15H4C4 1 x 50µg Anti-Cytochrome c monoclonal antibody clone 37BA11 1 x 50µg Detergent (0.1% (v/v) Triton® X-100 in PBS) 1 x 30ml Goat anti-mouse IgG2a - FITC secondary antibody 1 x 50µg Goat anti-mouse IgG2b - TXRD secondary antibody 1 x 50µg -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110417于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
图片
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Immunocytochemistry/ Immunofluorescence - ApoTrack™ Cytochrome c Apoptosis ICC Antibody Kit (ab110417)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab110417 被引用在 11 文献中.
- Pevná V et al. Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG Cells. Cells 12:N/A (2023). PubMed: 37408275
- Kang YJ et al. Administration of 4-hexylresorcinol increases p53-mediated transcriptional activity in oral cancer cells with the p53 mutation. Oncol Rep 48:N/A (2022). PubMed: 35856441
- Delahunty I et al. 7-Dehydrocholesterol Encapsulated Polymeric Nanoparticles As a Radiation-Responsive Sensitizer for Enhancing Radiation Therapy. Small 18:e2200710 (2022). PubMed: 35304816
- Lee C et al. Ultrasmall Gd@Cdots as a radiosensitizing agent for non-small cell lung cancer. Nanoscale 13:9252-9263 (2021). PubMed: 33982686
- Zheng Y et al. Pinocembrin induces ER stress mediated apoptosis and suppresses autophagy in melanoma cells. Cancer Lett 431:31-42 (2018). PubMed: 29807112