AB109909

Complex IV人Enzyme Activity Microplate Assay试剂盒

Name: Complex IV 人Enzyme Activity Microplate检测试剂盒 (ab109909)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109909
Price: 7489 CNY
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Complex IV Human Enzyme Activity Microplate Assay Kit

(2 Reviews)

(59 Publications)

Complex IV Human Enzyme Activity Microplate Assay Kit ab109909 is used to determine the activity of cytochrome c oxidase in human samples. The assay is based on antibody-based capture of Complex IV on the plate, followed by a biochemical assay.

Uses antibody capture for improved assay specificity

查看别名

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1

2 Images

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)

FuncS

Supplier Data

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)

Figure 1. To determine the activity in the sample, calculate the slope by using microplate software or by manual calculations using one of the two methods shown. Compare the sample rate with the rate of the control (normal) sample and with the rate of the null (background) to get the relative Complex IV activity. (A)The rate is determined by calculating the gradient of the initial slope over the linear region. (B) The rate is determined by calculating the slope between two points within the linear region.

FuncS

Supplier Data

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)

Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.

关键信息

检测方法

Colorimetric

样品类型

Cell culture extracts, Tissue

反应种属

Cow, Human, Pig

检测类型

Enzyme activity

检测平台

Microplate reader

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反应性数据

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产品详情

Complex IV Human Enzyme Activity Microplate Assay Kit ab109909 is used to determine the activity of cytochrome c oxidase in a human sample.

The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm.

Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.

Store Tube 1, detergent, and microplate at 4°C – DO NOT FREEZE. Store Reagent C at -20°C or at -80°C for long-term storage.

Range of complex IV / cytochrome c oxidase assay kits
Immunocapture with biochemical assay (plate-based) ab109911 and (this kit) ab109909.
Immunocapture with biochemical assay (dipstick) ab109878 and ab109876
Immunocapture with biochemical assay and ELISA ab109910

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规格

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab109909-96Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Reagent C (Reduced Cytochrome c)", "number":"AB109909-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-well microplate (12 strips)", "number":"AB109909-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"Detergent", "number":"AB109909-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"Tube 1 (Buffer)", "number":"AB109909-CMP03", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Dry Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complex IV also known as cytochrome c oxidase is an important component of the electron transport chain in mitochondria. This enzyme complex has significant mass approximately 204 kDa and operates primarily in the inner mitochondrial membrane. It catalyzes the transfer of electrons from cytochrome c to oxygen facilitating ATP generation. Complex IV is ubiquitously expressed in all tissues with higher expression in organs with high energy demands like heart and skeletal muscle. This enzyme is composed of multiple subunits encoded by both mitochondrial and nuclear DNA.

Biological function summary

Cytochrome c oxidase plays a critical role in cellular respiration. It forms part of the larger enzyme complex which also includes Complex I II and III. These complexes work together to create a proton gradient across the inner mitochondrial membrane essential for ATP synthesis via oxidative phosphorylation. This enzyme's activity is measured through assays like the cytochrome c oxidase assay which evaluates its function in various tissues. These assays help researchers understand how effectively electrons are being transferred and protons are driven across the membrane.

Pathways

Complex IV is integral to the oxidative phosphorylation pathway and the broader mitochondrial respiratory chain. It interacts closely with cytochrome c a small heme protein that shuttles electrons between Complex III (cytochrome c reductase) and Complex IV. Through these interactions the proton gradient is established enabling ATP synthase to convert chemical energy into usable cell energy. This process significantly impacts cellular metabolism and energy production influencing how efficiently cells function.

Complex IV dysfunction is associated with mitochondrial diseases and certain neurodegenerative disorders. For instance defects in cytochrome c oxidase lead to conditions like Leigh syndrome a severe neurological disorder. Also studies show that disruptions in the electron transport chain involving Complex IV relate closely to Alzheimer's disease. Alterations in proteins like cytochrome c which work in tandem with Complex IV can exacerbate these conditions highlighting the importance of this target in understanding and potentially treating these diseases.

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产品实验方案

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靶点信息

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX4I1

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文献 (59)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:407-442 PubMed39623165

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Mitochondrial calcium uniporter complex controls T-cell-mediated immune responses.

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Magdalena Shumanska,Dmitri Lodygin,Christine S Gibhardt,Christian Ickes,Ioana Stejerean-Todoran,Lena C M Krause,Kira Pahl,Lianne J H C Jacobs,Andrea Paluschkiwitz,Shuya Liu,Angela Boshnakovska,Niels Voigt,Tobias J Legler,Martin Haubrock,Miso Mitkovski,Gereon Poschmann,Peter Rehling,Sven Dennerlein,Jan Riemer,Alexander Flügel,Ivan Bogeski

Nature communications 15:9380 PubMed39477919

2024

A wearable and stretchable dual-wavelength LED device for home care of chronic infected wounds.

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Ming Li,Chenxi Wang,Qiang Yu,Haoyi Chen,Yingying Ma,Li Wei,Mei X Wu,Min Yao,Min Lu

Cell death & disease 15:407 PubMed38862500

2024

ER-mitochondria association negatively affects wound healing by regulating NLRP3 activation.

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Caterina Licini,Gianluca Morroni,Guendalina Lucarini,Veronica Angela Maria Vitto,Fiorenza Orlando,Sonia Missiroli,Gloria D'Achille,Mariasole Perrone,Tatiana Spadoni,Laura Graciotti,Giorgia Bigossi,Mauro Provinciali,Annamaria Offidani,Monica Mattioli-Belmonte,Oscar Cirioni,Paolo Pinton,Oriana Simonetti,Saverio Marchi

Pflugers Archiv : European journal of physiology 476:939-948 PubMed38446167

2024

Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.

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Ed Maunder,Andrew King,Jeffrey A Rothschild,Matthew J Brick,Warren B Leigh,Christopher P Hedges,Troy L Merry,Andrew E Kilding

Nature cell biology 25:1575-1589 PubMed37770567

2023

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

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Nicholas J Kramer,Gyan Prakash,R Stefan Isaac,Karine Choquet,Iliana Soto,Boryana Petrova,Hope E Merens,Naama Kanarek,L Stirling Churchman

Journal of dairy science 107:1197-1210 PubMed37709028

2023

Peripheral blood mononuclear cell mitochondrial enzyme activity in calves is associated with average daily gain, reproductive outcomes, lactation performance, and survival.

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A M Niesen,H A Rossow

Redox biology 66:102847 PubMed37597422

2023

The mitochondria-targeted sulfide delivery molecule attenuates drugs-induced gastropathy. Involvement of heme oxygenase pathway.

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Katarzyna Magierowska,Dagmara Wójcik-Grzybek,Edyta Korbut,Dominik Bakalarz,Grzegorz Ginter,Aleksandra Danielak,Sławomir Kwiecień,Anna Chmura,Roberta Torregrossa,Matthew Whiteman,Marcin Magierowski

Frontiers in molecular neuroscience 16:1201015 PubMed37614699

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Cell type and sex specific mitochondrial phenotypes in iPSC derived models of Alzheimer's disease.

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Kaitlin Flannagan,Julia A Stopperan,Brittany M Hauger,Benjamin R Troutwine,Colton R Lysaker,Taylor A Strope,Vivien Csikos Drummond,Caleb A Gilmore,Natalie A Swerdlow,Julia M Draper,Cynthia M Gouvion,Jay L Vivian,Mohammad Haeri,Russell H Swerdlow,Heather M Wilkins

Cell death & disease 13:988 PubMed36418320

2022

COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolism.

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Yetong Feng,Jiayi Xu,Mengjiao Shi,Rongrong Liu,Lei Zhao,Xin Chen,Miaomiao Li,Yaping Zhao,Jiahui Chen,Wenjing Du,Pengfei Liu

Cells 11: PubMed36139466

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Effects of Elamipretide on Autophagy in Renal Cells of Pigs with Metabolic Syndrome.

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Siting Hong,Ramyar Ghandriz,Sarosh Siddiqi,Xiang-Yang Zhu,Ishran M Saadiq,Kyra L Jordan,Hui Tang,Khaled A Ali,Amir Lerman,Alfonso Eirin,Lilach O Lerman

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Complex IV人Enzyme Activity Microplate Assay试剂盒

Complex IV Human Enzyme Activity Microplate Assay Kit

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)