AB109908

Complex II Enzyme Activity Microplate Assay试剂盒

Name: Complex II Activity检测试剂盒 (Colorimetric) (ab109908)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109908
Price: 6485 CNY
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Complex II Activity Assay Kit (Colorimetric)

(2 Reviews)

(125 Publications)

Complex II Activity Assay Kit (Colorimetric) ab109908 is designed for determining the Complex II activity in human, mouse, rat or bovine samples.

查看别名

SDH2, SDHF, SDHA, Flavoprotein subunit of complex II, Malate dehydrogenase [quinone] flavoprotein subunit, Fp

3 Images

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

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Supplier Data

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

This assay is compatible with different sample types such as mitochondria, tissue or cell lysates and in multiple species including human and rodent samples. Typical linear range data are shown for ab109908.

FuncS

Supplier Data

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

Example of raw data. Note the lag period before activity. Also note the activity of mitochondria (BHM, bovine heart mitochondria) is higher than whole cell lysate (HepG2, human hepatoblastoma) and the reaction ends at >1600 seconds because the substrates are used up.

FuncS

Supplier Data

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, substrate is added, and enzyme activity is analyzed by measuring the change in absorbance of either the substrate or the product of the reaction (depending upon which enzyme is being analyzed). By analyzing the enzyme's activity in an isolated context, outside of the cell and free from any other variables, an accurate measurement of the enzyme's functional state can be understood.

关键信息

检测方法

Colorimetric

样品类型

Tissue Lysate, Purified mitochondria, Cell Lysate

反应种属

Mouse, Rat, Cow, Human

检测类型

Enzyme activity

检测时间

检测平台

Microplate reader

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Complex II Activity Assay Kit (Colorimetric) ab109908 is designed for determining the Complex II activity in a human, mouse, rat or bovine sample. Each of the 96 wells in the kit has been coated with an anti-Complex II monoclonal antibody (mAb) which purifies the enzyme from a complex sample such as mitochondria, tissue homogenate or cell lysate. After this in-well purification the production of ubiquinol by the enzyme is coupled to the reduction of the dye DCPIP (2,6-diclorophenolindophenol) and a decreases in its absorbance at 600 nm, which in turn recycles the substrate ubiquinone.

Succinate, Ubiquinone 2, DCPIP and Phospholipids should be stored at -80°C. All other components should be stored at 4°C.

This product was previously called Complex II Enzyme Activity Microplate Assay Kit

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规格

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性能和储存信息

运输条件

Dry Ice

储存信息

Please refer to protocols

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complex II also known as succinate dehydrogenase (SDH) or succinate-ubiquinone oxidoreductase plays an important mechanical role in the mitochondrial electron transport chain. It catalyzes the oxidation of succinate to fumarate while reducing ubiquinone to ubiquinol. The enzyme complex is approximately 140 kDa in mass and resides in the inner mitochondrial membrane. Complex II is expressed in most tissues particularly in high-energy demand tissues such as the heart and skeletal muscles.

Biological function summary

Complex II functions as part of the larger electron transport chain complex and plays a role in the Krebs cycle. It links two critical metabolic pathways converting succinate to fumarate while transferring electrons to the electron transport chain. This makes it integral for proper cellular respiration and energy production. The complex consists of multiple subunits and utilizes co-factors like FAD and iron-sulfur clusters for enzymatic activity. It is also a part of the supercomplexes that optimize the efficiency of oxidative phosphorylation.

Pathways

Complex II plays a significant role in both the citric acid cycle and oxidative phosphorylation. It acts as a connecting bridge between these two pathways facilitating the flow of electrons. Complex II works alongside other proteins such as complex I and complex III to maintain the electron transport chain's function and energy production. Succinate dehydrogenase transfers electrons within the chain directly affecting the generation of ATP by complex V (ATP synthase).

Dysfunction of complex II is associated with mitochondrial diseases and cancers. Mutations or deficiencies in its subunits can lead to conditions like Leigh syndrome and hereditary paraganglioma. These conditions frequently involve other mitochondrial proteins and complexes such as complex I which can exacerbate the electron transport chain dysfunction. In cancers alterations in succinate dehydrogenase activity can result in oncogenic metabolisms by falsely stabilizing hypoxia-inducible factors linking it further with the genetic and metabolic regulation.

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产品实验方案

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靶点信息

Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed : 10746566, PubMed : 24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol-oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed : 20484225).

See full target information SDHA

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文献 (125)

Recent publications for all applications. Explore the full list and refine your search

GeroScience 47:5881-5901 PubMed40111699

2025

Chronic alcohol consumption accelerates cardiovascular aging and decreases cardiovascular reserve capacity.

Applications

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Species

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Partha Mukhopadhyay,Burhan Yokus,Bruno Paes-Leme,Sándor Bátkai,Zoltán Ungvári,György Haskó,Pal Pacher

The Journal of clinical investigation 135: PubMed39786963

2025

Loss of Cpt1a results in elevated glucose-fueled mitochondrial oxidative phosphorylation and defective hematopoietic stem cells.

Applications

Unspecified application

Species

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Jue Li,Jie Bai,Vincent T Pham,Michihiro Hashimoto,Maiko Sezaki,Qili Shi,Qiushi Jin,Chenhui He,Amy Armstrong,Tian Li,Mingzhe Pan,Shujun Liu,Yu Luan,Hui Zeng,Paul R Andreassen,Gang Huang

International journal of medical sciences 21:2464-2479 PubMed39439466

2024

BuyangHuanwu Decoction alleviates Endothelial Cell Apoptosis and Coronary Microvascular Dysfunction via Regulation of the MAPKK4/p38 Signaling Axis.

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Xing Chang,Dan Wu,Xin Gao,Jianguo Lin,Ying Tan,Junyan Wang,Hang Zhu,Hao Zhou

The EMBO journal 43:5941-5971 PubMed39420094

2024

An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease.

Applications

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Species

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Nan Zhao,Antonio Michelucci,Laura Pietrangelo,Sundeep Malik,Linda Groom,Jennifer Leigh,Thomas N O'Connor,Takahiro Takano,Paul D Kingsley,James Palis,Simona Boncompagni,Feliciano Protasi,Robert T Dirksen

Communications biology 7:1250 PubMed39363102

2024

Sexual dimorphism in a mouse model of Friedreich's ataxia with severe cardiomyopathy.

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Species

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Lili Salinas,Claire B Montgomery,Francisco Figueroa,Phung N Thai,Nipavan Chiamvimonvat,Gino Cortopassi,Elena N Dedkova

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402805 PubMed39119832

2024

Irisin-Encapsulated Mitochondria-Targeted Biomimetic Nanotherapeutics for Alleviating Acute Kidney Injury.

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Species

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Xia Zhang,Lijia Liang,Fengxian Wang,Pedro A Jose,Ken Chen,Chunyu Zeng

EBioMedicine 106:105268 PubMed39098108

2024

NR4A3 prevents diabetes induced atrial cardiomyopathy by maintaining mitochondrial energy metabolism and reducing oxidative stress.

Applications

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Species

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Hong Peng,Jiali Yuan,Zhengshuai Wang,Binfeng Mo,Yihui Wang,Yuepeng Wang,Qunshan Wang

Cell communication and signaling : CCS 22:336 PubMed38898530

2024

SHH induces macrophage oxidative phosphorylation and efferocytosis to promote scar formation.

Applications

Unspecified application

Species

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Julei Zhang,Zeliang He,Chenlu Xiong,Yuanyuan Yao,Chengliang Zhang,Wende Yao,Sihan Yang,Xiaodong Li,Yan Han

Nature communications 15:4195 PubMed38760351

2024

Ferritinophagy mediates adaptive resistance to EGFR tyrosine kinase inhibitors in non-small cell lung cancer.

Applications

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Species

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Hui Wang,Qianfan Hu,Yuzhong Chen,Xing Huang,Yipeng Feng,Yuanjian Shi,Rutao Li,Xuewen Yin,Xuming Song,Yingkuan Liang,Te Zhang,Lin Xu,Gaochao Dong,Feng Jiang

Phytotherapy research : PTR 38:2496-2517 PubMed38447978

2024

Quercetin inhibits necroptosis in cardiomyocytes after ischemia-reperfusion via DNA-PKcs-SIRT5-orchestrated mitochondrial quality control.

Applications

Unspecified application

Species

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Xing Chang,Qin Zhang,Yu Huang,Jinfeng Liu,Yanli Wang,Xuanke Guan,Qiaomin Wu,Zhiming Liu,Ruxiu Liu

View all publications

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websiteProtocolBooklet

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Complex II Enzyme Activity Microplate Assay试剂盒

Complex II Activity Assay Kit (Colorimetric)

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)