Chemiluminescent HRP底物(membrane) (ab5801)
概述
-
产品名称
Chemiluminescent HRP底物(membrane) -
产品概述
A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.
The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities
-
说明
Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.
Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.
-
经测试应用
适用于: WBmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 1 kit luminol reagent. 1 x 20ml reaction buffer. 2 x 20ml -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
Incubate membrane in chemiluminescent substrate for 1 minute then rinse off. |
说明 |
---|
WB
Use at an assay dependent concentration. Incubate membrane in chemiluminescent substrate for 1 minute then rinse off. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab5801 被引用在 2 文献中.
- Wang P et al. Decorin reduces hypertrophic scarring through inhibition of the TGF-ß1/Smad signaling pathway in a rat osteomyelitis model. Exp Ther Med 12:2102-2108 (2016). WB . PubMed: 27698699
- Pacini S et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). PubMed: 20360837