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AB5801

Chemiluminescent HRP底物(membrane)

Chemiluminescent HRP Substrate (membrane)

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(2 Publications)

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

关键信息

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.</p>" } } }

产品详情

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab5801-1Kit", "assetComponentDetails": [ { "size":"2 x 20 mL", "name":"reaction buffer.", "number":"AB5801-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"luminol reagent.", "number":"AB5801-CMP01", "productcode":"" } ] } } }

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
+4°C

产品实验方案

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:2102-2108 PubMed27698699

2016

Decorin reduces hypertrophic scarring through inhibition of the TGF-β1/Smad signaling pathway in a rat osteomyelitis model.

Applications

WB

Species

Unspecified reactive species

Peng Wang,Xiangyan Liu,Peng Xu,Jialiang Lu,Runze Wang,Weidong Mu

PloS one 5:e9861 PubMed20360837

2010

Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs).

Applications

Unspecified application

Species

Unspecified reactive species

Simone Pacini,Vittoria Carnicelli,Luisa Trombi,Marina Montali,Rita Fazzi,Edoardo Lazzarini,Stefano Giannotti,Mario Petrini
View all publications

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