Ceruloplasmin Activity Assay试剂盒(Colorimetric) (ab273296)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Serum
概述
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产品名称
Ceruloplasmin Activity Assay试剂盒(Colorimetric)
参阅全部 Ceruloplasmin 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum -
检测类型
Enzyme activity (quantitative) -
范围
5 mU/ml - 500 mU/ml -
实验步骤
Multiple steps standard assay -
产品概述
Ceruloplasmin Activity Assay Kit (Colorimetric) (ab273296) utilizes an oxidase substrate, which gives an intensely colored product upon oxidation. The kit is fast, easy to use and suitable for high throughput applications and can measure ceruloplasmin activity in serum in the range between approximately 5 and 500 mU/ml.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K669 Ceruloplasmin Activity Colorimetric Assay Kit. K669-100 is the same size as the 100 test size of ab273296.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Ammonium Sulfate 1 x 10ml Ceruloplasmin Assay Buffer 1 x 25ml Ceruloplasmin Substrate 1 x 1ml H2O2 Standard 1 x 100µl Oxidizer I 1 x 100µl -
研究领域
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功能
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
组织特异性
Expressed by the liver and secreted in plasma. -
疾病相关
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
序列相似性
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- CERU_HUMAN
- Ceruloplasmin
- CP
see all
图片
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Typical Standard Curve of chemically oxidized substrate.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Typical Standard Curve of chemically oxidized substrate. The Standard Curve slope (for the points below 1.0 OD) is 0.0214 OD/nmol.
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Ceruloplasmin Activity AssayKinetic curves obtained from varying sample volumes of human serum. The kinetic slope for a 10 μl sample of an Ammonium Sulfate precipitate of frozen pooled human serum is 0.0189. Serum Ceruloplasmin Activity = 0.0189 (OD/min)/0.0214 (OD/nmol)/0.01ml X 2 = 176 mU/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273296 尚未被引用在任何文献中。