Cell Proliferation Staining试剂- Green Fluorescence - Cytopainter (ab176735)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Microplate reader, Flow cytometer
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Cell Proliferation Staining试剂- Green Fluorescence - Cytopainter
参阅全部 Cell viability/proliferation 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (quantitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. The CytoLabeling Green Reagent is designed to label cells at Em = 511-525 nm excited with a blue laser at Ex = 488 nm.
The CytoLabeling Green Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used.
Main features of CytoLabeling Green Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide
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说明
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
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平台
Microplate reader, Flow cytometer
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 500 tests 1000 tests CytoLabeling Green Reagent 1 vial 2 vials -
研究领域
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相关性
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
别名
- Cell Cytotoxicity
- cell tracking
图片
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Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735)
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735).
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Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735)Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735) and CFSE. Jurkat cells (2x106 cells/mL) were stained with ab176735 or CFSE (0.5 µM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.
数据表及文件
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Datasheet download
文献 (7)
ab176735 被引用在 7 文献中.
- Steinberg E et al. A fully 3D-printed versatile tumor-on-a-chip allows multi-drug screening and correlation with clinical outcomes for personalized medicine. Commun Biol 6:1157 (2023). PubMed: 37957280
- Alshafie W et al. Identification of high-performing antibodies for Moesin for use in Western Blot, immunoprecipitation, and immunofluorescence. F1000Res 12:172 (2023). PubMed: 38106655
- Akpe V et al. Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems. Assay Drug Dev Technol 19:191-203 (2021). PubMed: 33471566
- Lester Sequiera G et al. Generation and Evaluation of Isogenic iPSC as a Source of Cell Replacement Therapies in Patients with Kearns Sayre Syndrome. Cells 10:N/A (2021). PubMed: 33807701
- Akpe V et al. Batch Effect Adjustment to Lower the Drug Attrition Rate of MCF-7 Breast Cancer Cells Exposed to Silica Nanomaterial-Derived Scaffolds. Assay Drug Dev Technol 19:46-61 (2021). PubMed: 33443468
- Yahn SL et al. Fibrotic scar after experimental autoimmune encephalomyelitis inhibits oligodendrocyte differentiation. Neurobiol Dis 134:104674 (2020). PubMed: 31731043
- Nassour J et al. Autophagic cell death restricts chromosomal instability during replicative crisis. Nature 565:659-663 (2019). PubMed: 30675059
