Cell Cycle Assay试剂盒(Fluorometric - Red) (ab112117)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Cell Cycle Assay试剂盒(Fluorometric - Red)
参阅全部 Cell Cycle 试剂盒 -
检测方法
Fluorescent -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based (quantitative) -
种属反应性
与反应: Mammals, Other species -
产品概述
ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).
Visit our FAQs page for tips and troubleshooting. -
说明
Store ab112117 desiccated.
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平台
Flow cytometer
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 100X RNase A 1 x 250µl Assay Buffer 1 x 50ml Nuclear Red CCS1 1 x 250µl -
研究领域
图片
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)DNA profile in growing Jurkat cells.
Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab112117 被引用在 2 文献中.
- Arabsorkhi Z et al. miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN. J Cell Physiol 235:4335-4350 (2020). PubMed: 31621072
- Parada CA et al. Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression. Sci Rep 8:2098 (2018). Functional Studies . PubMed: 29391485