AB65302

Cathepsin D Activity Assay试剂盒(Fluorometric)

Name: Cathepsin D Activity检测试剂盒 (Fluorometric) (ab65302)| Abcam中文官网
Brand: Abcam Limited
SKU: AB65302
Price: 6337 CNY
Availability: InStock
Rating: 4 (3 reviews)

Cathepsin D Activity Assay Kit (Fluorometric)

(3 Reviews)

(142 Publications)

Cathepsin D Activity Assay Kit (Fluorometric) ab65302 uses a substrate based on the GKPILFFRLK(Dnp)-D-R-NH2) Cathepsin D substrate. Add reaction mix to samples and incubate for 1-2 hr at 37°C. Readout on any fluorometric (Ex/Em 328/460 nm) plate reader.

- Cited in > 120 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

6 Images

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

FuncS

Lab

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Cathepsin D measured in mouse tissue lysates (mg of extracted protein), background signal subtracted (duplicates +/- SD).

FuncS

Supplier Data

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Cathepsin D measured in Jurkat cells.

FuncS

Lab

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Cathepsin D levels were measured in standard control samples from ab119586; background signal subtracted (duplicates +/- SD).

FuncS

Lab

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Cathepsin D measured in cell lysates, background signal subtracted (duplicates +/- SD).

FuncS

PubMed

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Enzyme activity of Cathepsin B and Cathepsin D was determined using Cathepsin B/D activity assay kit (ab65300 and ab65302). Neuro2a cell lysates were centrifuged at 10,000 xg for 10 minutes at 4°C and supernatant was used for the assay. Cathepsin activity was performed at the 24 and 48 hours time points by cleavage of the fluorescence peptide substrate [DnP-DR-MCA, GKPILFFRLK(DnP)-DR substrate peptide labeled with MCA]. Data represent the mean ± SD (*p<0.05, **p<0.001).

Bong G.J., PLoS One 8(10), Fig 5F. doi:10.1371/journal.pone.0076466 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Schematic Diagram - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Schematic Diagram

Supplier Data

Schematic Diagram - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Representative image of Cathepsin D Activity Assay Kit (Fluorometric) ab65302

Components shown from left to right :

- Substrate II

- CD Reaction Buffer

- CD Cell Lysis Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

检测方法

Fluorescent

样品类型

Tissue Extracts, Cell Lysate

检测类型

Semi-quantitative

检测时间

检测平台

Microplate reader

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Cathepsin D Activity Assay Kit (Fluorometric) ab65302 is a fluorescence-based assay to measure Cathepsin D.

How the assay works

Cathepsin D Activity Assay Kit (Fluorometric) utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA.

Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.

Cathepsin D assay protocol summary:

-Add samples to wells
- Add reaction mix and incubate for 1-2 hr at 37°C
- Measure fluorescence (Ex/Em = 328/460 nm)

Related and recommended products

See below other alternative Cathepsin Assay Kits:
- Cathepsin L Assay Kit (Magic Red) ab270774
- Cathepsin K Assay Kit (Magic Red) ab270774

This product is manufactured by BioVision, an Abcam company and was previously called K143 Cathepsin D Activity Fluorometric Assay Kit. K143-100 is the same size as the 100 test size of ab65302.

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

规格

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab65302-2000Test", "assetComponentDetails": [ { "size":"20 x 0.2 mL", "name":"Substrate II", "number":"AB65302-CMP03" }, { "size":"20 x 100 mL", "name":"CD Cell Lysis Buffer", "number":"AB65302-CMP01" }, { "size":"20 x 5 mL", "name":"CD Reaction Buffer", "number":"AB65302-CMP02" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab65302-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"Substrate II", "number":"AB65302-CMP03", "productcode":"" }, { "size":"1 x 100 mL", "name":"CD Cell Lysis Buffer", "number":"AB65302-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"CD Reaction Buffer", "number":"AB65302-CMP02", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.

Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.

style

left-column

产品实验方案

style

left-column

靶点信息

See full target information Cathepsin D

style

left-column

文献 (142)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurobiology 62:13786-13806 PubMed40588669

2025

UQCRC1 is a Key Pathogenic Determinant and Potential Therapeutic Target for Cognitive Impairment in Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Zuoxi Wu,Zonghong Long,Ceng Feng,Fuhai Bai,Hong Li

Science advances 11:eads3393 PubMed40397740

2025

Neuronal lipofuscinosis caused by Kufs disease/CLN4 DNAJC5 mutations but not by a CSPα/DNAJC5 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago López-Begines,Nozha Borjini,Ángela Lavado-Roldán,Cristina Mesa-Cruz,Fabiola Mavillard,Vera I Wiersma,Fátima Rubio-Pastor,Emanuela Tumini,Carmen Paradela-Leal,María L Chiclana-Valcárcel,Carolina Aguado,Rafael Luján,Wiep Scheper,José L Nieto-González,Rafael Fernández-Chacón

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70046 PubMed40339170

2025

Hyperactivation of RAB5 disrupts the endosomal Rab cascade leading to endolysosomal dysregulation in Down syndrome: A necessary role for increased APP gene dose.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Qiao Chen,Xinxin Zuo,Ann Becker,William C Mobley

Molecular neurodegeneration 20:45 PubMed40269985

2025

Increased TMEM106B levels lead to lysosomal dysfunction which affects synaptic signaling and neuronal health.

Applications

Unspecified application

Species

Unspecified reactive species

Jolien Perneel,Miranda Lastra Osua,Sara Alidadiani,Nele Peeters,Linus De Witte,Bavo Heeman,Simona Manzella,Riet De Rycke,Mieu Brooks,Ralph B Perkerson,Elke Calus,Wouter De Coster,Manuela Neumann,Ian R A Mackenzie,Debby Van Dam,Bob Asselbergh,Tommas Ellender,Xiaolai Zhou,Rosa Rademakers

Translational psychiatry 15:126 PubMed40185739

2025

Pitolisant alleviates brain network dysfunction and cognitive deficits in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zou,Linhan Yang,Jiahui Zhu,Jihua Fan,Hanrun Zheng,Xiang Liao,Zhiqi Yang,Kuan Zhang,Hongbo Jia,Arthur Konnerth,Yan-Jiang Wang,Chunqing Zhang,Yun Zhang,Sunny C Li,Xiaowei Chen

Journal of neuroinflammation 22:68 PubMed40055725

2025

Microglial progranulin differently regulates hypothalamic lysosomal function in lean and obese conditions via cleavage-dependent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Chae Beom Park,Chan Hee Lee,Gil Myoung Kang,Se Hee Min,Min-Seon Kim

MedComm 6:e70075 PubMed39968501

2025

Histidine triad nucleotide-binding protein 2 attenuates doxorubicin-induced cardiotoxicity through restoring lysosomal function and promoting autophagy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Jiang,Jinyan Zhang,Daile Jia,Liwei Liu,Jinfeng Gao,Beijian Zhang,Zhen Dong,Xiaolei Sun,Wenlong Yang,Tiantong Ou,Suling Ding,Luna He,Yiqin Shi,Kai Hu,Aijun Sun,Junbo Ge

Viruses 17: PubMed39861814

2025

Activity of Various Cathepsin Proteases and Enrichment of Klotho Protein in the Urine and Urinary Extracellular Vesicles After SARS-CoV-2 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Niharika Bala,Ramish H Rafay,Sarah C Glover,Abdel A Alli

The Journal of clinical investigation 135: PubMed39724071

2024

Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Saarela,Pawel Lis,Sara Gomes,Raja S Nirujogi,Wentao Dong,Eshaan Rawat,Sophie Glendinning,Karolina Zeneviciute,Enrico Bagnoli,Rotimi Fasimoye,Cindy Lin,Kwamina Nyame,Fanni A Boros,Friederike Zunke,Frederic Lamoliatte,Sadik Elshani,Matthew Jaconelli,Judith Jm Jans,Margriet A Huisman,Christian Posern,Lena M Westermann,Angela Schulz,Peter M van Hasselt,Dario R Alessi,Monther Abu-Remaileh,Esther M Sammler

The Journal of international medical research 52:3000605241301877 PubMed39612318

2024

A novel homozygous mutation identified by whole exome sequencing in a consanguineous family with metachromatic leukodystrophy: a case report.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyi Li,Xiaoni Kuang,Guangwen Huang,Zhenyu Liu,Shuyuan Yan

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cathepsin D Activity Assay试剂盒(Fluorometric)

Cathepsin D Activity Assay Kit (Fluorometric)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Functional Studies - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)

Schematic Diagram - Cathepsin D Activity Assay Kit (Fluorometric) (AB65302)