Caspase-10 (active) Staining试剂盒- Green Fluorescence

产品详情

Caspase 10 (active) Staining Kit - Green Fluorescence (ab219937) is a sensitive fluorometric assay to measure caspase 10 activation in live cells. The assay uses FAM-AEVD-FMK, which binds irreversibly to active caspase 10 in apoptotic cells. The fluorescent intensity of the FAM-AEVD-FMK signal is proportional to the amount of active caspase 10 and can be easily detected at Ex/Em = 490/525 nm by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.

Caspase activity assay kits are based on fluorescent inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. Caspase 10 plays an important role in death receptor signaling and apoptosis induction. It has been proven that caspase 10 has substrate selectivity for the peptide sequence Ala-Glu-Val-Asp (AEVD). This kit uses FAM-AEVD-FMK as a fluorescent indicator for caspase 10 activity. FAM-AEVD-FMK irreversibly binds to activated caspase 10 in apoptotic cells. Once bound to caspase 10, the fluorescent reagent is retained inside the cell. The binding event inhibits caspase 10 but will not stop apoptosis from proceeding.