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AB325200

BOND RX - Anti-CD276 antibody [EPR27429-173]

BOND RX - Anti-CD276 antibody [EPR27429-173]

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Rabbit Recombinant Monoclonal CD276 antibody. Suitable for IHC-P and reacts with Human, Mouse samples. This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-CD276 antibody [EPR27429-173] - ab313380.

- Validated on the Leica BOND RX automated IHC staining platform for CD276 IHC
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency

查看别名

CD276, B7h3, Cd276, CD276 antigen, B7 homolog 3, Costimulatory molecule, B7-H3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-CD276 antibody [EPR27429-173] (AB325200)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOND RX - Anti-CD276 antibody [EPR27429-173] (AB325200)

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue labelling CD276 with ab313380 [EPR27429-173] at 0.5 µg/mL concentration.

All steps were performed at room temperature unless otherwise stated.

Staining was performed on the BOND RX Advanced Staining Instrument from Leica Biosystems, using the Bond Polymer Refine Detection kit (DS9800) and the following protocols :

  1. Deparaffinization - Dewax
  2. Antigen retrieval - HIER with ER2 (pH 9.0), 20 minutes, 100°C
  3. Staining – modified IHC Protocol F (Marker : ab313380 for 15 minutes with 3 x Bond Wash Solution steps) where standard post-primary step has been excluded.

Sections were counterstained with Hematoxylin (DS9800).

Secondary Antibody only control was performed with the same staining protocols, with marker as negative.

关键信息

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.5 µg/mL", "notes":"<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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产品详情

This product is a bundle of 2 x BOND Titration Containers (with 6mL inserts) along with 100 uL vial of Anti-CD276 antibody [EPR27429-173] - ab313380.

Additional Titration Inserts can be purchased from Leica Biosystems, using OPT9719 (box of 50). Or Titration Container/Insert Kit, using OPT9049 (box of 10/50).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab325200-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Anti-CD276 antibody [EPR27429-173]", "number":"AB325200-CMP02", "productcode":"AB313380" }, { "size":"2 x 1 Unit", "name":"LBS BOND RX titration container", "number":"AB325200-CMP01", "productcode":"" } ] } } }
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.
See full target information Cd276
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websiteProtocolBooklet
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