beta-Glucosidase Assay试剂盒(ab272521)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
概述
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产品名称
beta-Glucosidase Assay试剂盒 -
检测方法
Colorimetric -
检测类型
Quantitative -
范围
2 mU/ml - 250 mU/ml -
产品概述
Beta-Glucosidase Assay Kit (ab272521) is a simple, direct and automation-ready procedure for measuring β-glucosidase activity. beta-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
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经测试应用
适用于: Functional Studiesmore details -
平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests β-NPG Substrate 1 x 1ml Assay Buffer 1 x 24ml Calibrator 1 x 10ml
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272521于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of beta-Glucosidase standard curve.
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Progress CurvesExample of beta-Glucosidase reaction progress curves.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272521 被引用在 1 文献中.
- Perez-Abshana LP et al. Rotenone Blocks the Glucocerebrosidase Enzyme and Induces the Accumulation of Lysosomes and Autophagolysosomes Independently of LRRK2 Kinase in HEK-293 Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37445771