ATPase/GTPase Assay试剂盒(ab272520)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
概述
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产品名称
ATPase/GTPase Assay试剂盒 -
检测方法
Colorimetric -
检测类型
Quantitative -
产品概述
ATPase/GTPase Assay Kit (ab272520) offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).
High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes.
Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
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经测试应用
适用于: Functional Studiesmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 200 tests 200 tests Assay Buffer 1 x 10ml 1 x 10ml Pi Standard (1 mM) 1 x 1ml 1 x 1ml Reagent 1 x 50ml 1 x 50ml
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example phosphate standard curve.
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Assay performancePhosphate assays in 96-well format.
1. H2O, 2. Phosphate, 3. ATP in H2O, 4. ATP/Phosphate in H2O, 5, 7, 9, 11, 13: ATP in Assay Buffer with, where indicated, 0.5 mg/mL BSA, 1 mM DTT, 2 mM β-mercaptoethanol (βME) and 5% DMSO. 6, 8, 10, 12, 14: ATP/Phosphate in Assay Buffer. Phosphate and ATP were at 50 µM and 1 mM, respectively. The assay is not affected by these component.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272520 被引用在 1 文献中.
- Vadla GP et al. ABT1 modifies SMARD1 pathology via interactions with IGHMBP2 and stimulation of ATPase and helicase activity. JCI Insight 8:N/A (2023). PubMed: 36480289