ATP synthase Enzyme Activity Microplate Assay试剂盒

• FuncS

PubMed

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

Mitochondrial ROS dependent functional deficiency and structural impairment in cardiac mitochondria after sepsis.

Mitchondiral fractions from the heart tissue of Rats infected by S. pneumoniae, or given PBS sham control, were subjected to measurements of complex I-V activities. Complex I was measured with ab109721 (top left), Complex II + III were measured using ab109905 (top right), Complex IV was measured using ab109911 (bottom left) and Complex V was measured using ab109714 (bottom right).

Freshly isolated mitochondrial pellets were resuspened in PBS supplemented with 10% detergent provided in the kits. Protein concentrations of these mitochondrial lysates were estimated and 25 μg (for complex I, IV and V) or 100 μg (for complex II+III) mitochondrial protein was used per reaction. Enzyme activities were measured spectrophotometricly in triplicate and expressed as changes of absorbance per minute per mg protein.

Image courtesy of Yao X et al.PLoS One. 2015; 10(10): e0139416. doi: 10.1371/journal.pone.0139416.

• FuncS

Supplier Data

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

Functional study using ATP synthase Activity Assay Kit (ab109714).

• FuncS

Supplier Data

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

Principle of ATP synthase Enzyme Activity Microplate Assay Kit (ab109714).

• FuncS

Supplier Data

Functional Studies - ATP synthase Activity Assay Kit (Colorimetric) (AB109714)

Examples of activity/load relationships for cultured cell lysate, rat liver mitochondria, and bovine heart mitochondria.