Asparagine Assay试剂盒(Fluorometric) (ab273333)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Cell culture media, Other biological fluids, Suspension cells
概述
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产品名称
Asparagine Assay试剂盒(Fluorometric) -
检测方法
Fluorescent -
样品类型
Other biological fluids, Adherent cells, Suspension cells, Cell culture media -
检测类型
Quantitative -
实验步骤
Multiple steps standard assay -
产品概述
Asparagine Assay Kit (Fluorometric) (ab273333) allows for highly sensitive quantification of L-asparagine levels in biological fluids and cultured cells.
In the assay, L-asparagine is hydrolyzed to generate aspartate, which is converted to pyruvate and subsequently reacts with a fluorogenic probe to form a stable fluorophore (Ex/Em = 538/587 nm) via a series of coupled enzymatic reactions. The assay is not affected by physiological concentrations of other common amino acids and can detect asparagine levels down to 5 μM in samples. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K736 Asparagine Assay Kit (Colorimetric). K736-100 is the same size as the 100 test size of ab273333.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer LII 1 x 25ml Asparagine Enzyme Mix 1 vial Conversion Enzyme Mix 1 vial Converter Enzyme XII 1 vial L-Asparagine Standard 1 vial OxiRed Probe 1 x 200µl Sample Clean-Up Mix 1 vial
图片
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Example data
Example data.
L-Asparagine Standard curve.
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Example dataExample data.
Specificity for detection of L-asparagine (ASN) over other common L-amino acids. At a 5- fold molar excess (10 nmole/well) versus ASN (2 nmole/well), tryptophan (TRP) gives ~5% interference and the reduced form of cysteine (CYS) gives ~20% interference (the oxidized disulfide form, which accounts for 90% of the total cysteine in biological fluids, generates ≤1% interference at 5-fold excess).
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Example dataExample data.
Estimation of total asparagine in pooled heparinized human plasma (10μl), pooled off-the-clot human serum (10μl) and pooled human CSF (15μl). Asparagine concentrations for plasma, serum and CSF samples were 25.61 ± 2.51 μM, 37.99 ± 1.77 μM and 11.11 ± 2.59 μM, respectively. Data are mean ± SEM of at least 3 replicates, samples were treated with Sample Cleanup Mix, deproteinized using 10 kDa spin columns and assayed according to the kit protocol.
数据表及文件
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SDS download
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Datasheet download
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