Asparaginase Activity Assay试剂盒(ab107922)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Assay time: 40 min
- Sample type: Cell Lysate
概述
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产品名称
Asparaginase Activity Assay试剂盒 -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell Lysate -
检测类型
Enzyme activity -
检测时间
0h 40m -
产品概述
Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.
In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
Asparaginase assay protocol summary:
- add reaction mix to sample and standard wells, mix and incubate for 10 min
- analyze with microplate reader in kinetic mode for 30-60 min -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K754 Asparaginase Activity Colorimetric/Fluorometric Assay Kit. K754-100 is the same size as the 100 test size of ab107922.
Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Asparaginase Positive Control 1 vial Aspartate Enzyme Mix 1 vial Aspartate Standard 1 x 0.1ml Assay Buffer IV 1 x 25ml Converter Enzyme XII 1 vial OxiRed Probe 1 x 0.2ml Asparaginase Substrate Mix 1 vial -
研究领域
图片
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Functional Studies - Asparaginase Activity Assay Kit (Colorimetric)
Typical asparaginase standard calibration curve using colorimetric reading.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Typical asparaginase standard calibration curve using fluorometric reading.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.
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Functional Studies - Asparaginase Activity Assay Kit (Fluorometric)Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab107922 被引用在 6 文献中.
- Biswas P et al. A Mouse Model with Ablated Asparaginase and Isoaspartyl Peptidase 1 (Asrgl1) Develops Early Onset Retinal Degeneration (RD) Recapitulating the Human Phenotype. Genes (Basel) 13:N/A (2022). PubMed: 36011372
- Stentz R et al. The Proteome of Extracellular Vesicles Produced by the Human Gut Bacteria Bacteroides thetaiotaomicron In Vivo Is Influenced by Environmental and Host-Derived Factors. Appl Environ Microbiol 88:e0053322 (2022). PubMed: 35916501
- Lin HC et al. Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera. Front Microbiol 11:573907 (2020). PubMed: 33193181
- Kim K et al. Cell mass-dependent expression of an anticancer protein drug by tumor-targetedSalmonella. Oncotarget 9:8548-8559 (2018). PubMed: 29492216
- Biswas P et al. A missense mutation in ASRGL1 is involved in causing autosomal recessive retinal degeneration. Hum Mol Genet 25:2483-2497 (2016). PubMed: 27106100
- Kim K et al. L-Asparaginase delivered by Salmonella typhimurium suppresses solid tumors. Mol Ther Oncolytics 2:15007 (2015). PubMed: 27119104