AB107922

Asparaginase Activity Assay试剂盒

Name: Asparaginase Activity检测试剂盒 (ab107922)| Abcam中文官网
Brand: Abcam Limited
SKU: AB107922
Price: 6406 CNY
Availability: InStock

Asparaginase Activity Assay Kit

Be the first to review this product! Submit a review

(6 Publications)

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) has one reagent addition step, followed by analysis with a colorimetric plate reader in kinetic mode for 30-60 min.

查看别名

b1767, JW1756, ansA, L-asparaginase 1, L-asparaginase I, L-asparagine amidohydrolase I, L-ASNase I

4 Images

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.

FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Colorimetric).

Typical asparaginase standard calibration curve using colorimetric reading.

FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.

FuncS

Lab

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (Fluorometric).

Typical asparaginase standard calibration curve using fluorometric reading.

关键信息

检测方法

Colorimetric/Fluorometric

样品类型

Cell Lysate

检测类型

Enzyme activity

检测时间

40m

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

产品详情

Asparaginase Activity Assay Kit (Colorimetric/Fluorometric) (ab107922) provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples.

Asparaginase assay principle
In the asparaginase assay protocol, asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected indirectly colorimetrically (OD=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.

Asparaginase assay protocol summary
- add reaction mix to sample and standard wells and mix
- analyze with microplate reader in kinetic mode for 30-60 min at room temperature

Other notes
This product was previously called K754 Biovision Asparaginase Activity Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs).

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

规格

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab107922-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Converter Mix J", "number":"AB107922-CMP04", "productcode":"" }, { "size":"1 x 0.1 mL", "name":"Aspartate Standard", "number":"AB107922-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Aspartate Enzyme Mix", "number":"AB107922-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Asparaginase Positive Control", "number":"AB107922-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 4", "number":"AB107922-CMP01", "productcode":"AB323949" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB107922-CMP05", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"Asparaginase Substrate Mix", "number":"AB107922-CMP07", "productcode":"" } ] } } }

style

left-column

性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Asparaginase commonly known as L-asparaginase is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. This enzyme is present in various organisms including bacteria such as Escherichia coli. It is often produced using recombinant methods in strains like E. coli as L-asparaginase or trade names like aspergcel. The molecular mass of L-asparaginase is approximately 135 kDa. It is primarily expressed in microbial cells but is also significant in commercial and medical applications for its enzymatic properties.

Biological function summary

This enzyme plays an important role in the metabolism of amino acids. Unlike humans and some tumor cells normal cells can synthesize L-asparagine while certain types of cancer cells lack this ability and depend on external sources. This dependency makes L-asparaginase an effective therapeutic enzyme as it depletes circulating L-asparagine inhibiting protein synthesis in such tumor cells. The enzyme functions independently and does not typically combine into complex structures.

Pathways

L-asparaginase is involved in the asparagine degradation pathway. This pathway impacts protein synthesis and nitrogen metabolism. When applied therapeutically it interacts indirectly with protein synthesis pathways by reducing the availability of asparagine for cells that cannot synthesize it. Additionally when pegylated PEG-asparaginase it displays enhanced stability and a prolonged circulating half-life providing an efficient effect on target pathways.

Asparaginase is prominently linked to the treatment of acute lymphoblastic leukemia (ALL). Its mechanism to lower asparagine levels starves leukemia cells of necessary nutrients leading to apoptosis. The enzyme is also studied for its potential effects in certain solid tumors that express deficiencies in asparagine synthetase a related protein. Moreover levels of asparaginase activity are often monitored via specialized assays to ensure therapeutic efficacy and manage side effects during treatment.

style

left-column

产品实验方案

style

left-column

靶点信息

See full target information ansA

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Genes 13: PubMed36011372

2022

A Mouse Model with Ablated Asparaginase and Isoaspartyl Peptidase 1 () Develops Early Onset Retinal Degeneration (RD) Recapitulating the Human Phenotype.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Pooja Biswas,Anne Marie Berry,Qais Zawaydeh,Dirk-Uwe G Bartsch,Pongali B Raghavendra,J Fielding Hejtmancik,Naheed W Khan,S Amer Riazuddin,Radha Ayyagari

Applied and environmental microbiology 88:e0053322 PubMed35916501

2022

The Proteome of Extracellular Vesicles Produced by the Human Gut Bacteria Bacteroides thetaiotaomicron Is Influenced by Environmental and Host-Derived Factors.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Régis Stentz,Emily Jones,Rokas Juodeikis,Udo Wegmann,Maria Guirro,Andrew J Goldson,Arlaine Brion,Catherine Booth,Padhmanand Sudhakar,Ian R Brown,Tamás Korcsmáros,Simon R Carding

Frontiers in microbiology 11:573907 PubMed33193181

2020

Diverse Enzymes With Industrial Applications in Four Thraustochytrid Genera.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiu-Chin Lin,Wei-Hao Li,Chi-Chih Chen,Tien-Hsing Cheng,Yu-Hsuan Lan,Ming-Der Huang,Wen-Ming Chen,Jo-Shu Chang,Hsin-Yang Chang

Oncotarget 9:8548-8559 PubMed29492216

2018

Cell mass-dependent expression of an anticancer protein drug by tumor-targeted .

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Kwangsoo Kim,Sa-Young Min,Ho-Dong Lim,Sung-Hwan You,Daejin Lim,Jae-Ho Jeong,Hyun-Ju Kim,Joon Haeng Rhee,Kyeongil Park,Minsang Shin,Geun-Joong Kim,Jung-Joon Min,Hyon E Choy

Human molecular genetics 25:2483-2497 PubMed27106100

2016

A missense mutation in ASRGL1 is involved in causing autosomal recessive retinal degeneration.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Pooja Biswas,Venkata Ramana Murthy Chavali,Giulia Agnello,Everett Stone,Christina Chakarova,Jacque L Duncan,Chitra Kannabiran,Melissa Homsher,Shomi S Bhattacharya,Muhammad Asif Naeem,Adva Kimchi,Dror Sharon,Takeshi Iwata,Shaikh Riazuddin,G Bhanuprakash Reddy,J Fielding Hejtmancik,George Georgiou,S Amer Riazuddin,Radha Ayyagari

Molecular therapy oncolytics 2:15007 PubMed27119104

2015

L-Asparaginase delivered by Salmonella typhimurium suppresses solid tumors.

Applications

Enzyme activity assay, Enzyme activity assay

Species

Escherichia coli, Saccharomyces cerevisiae

Kwangsoo Kim,Jae Ho Jeong,Daejin Lim,Yeongjin Hong,Hyung-Ju Lim,Geun-Joong Kim,So-Ra Shin,Je-Jung Lee,Misun Yun,Robert A Harris,Jung-Joon Min,Hyon E Choy

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Asparaginase Activity Assay试剂盒

Asparaginase Activity Assay Kit

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (AB107922)

Functional Studies - Asparaginase Activity Assay Kit (AB107922)