Aromatase (CYP19A)抑制剂Screening试剂盒(Fluorometric) (ab284522)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader, Fluorescence microscope
- Sample type: Inhibitor compounds
概述
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产品名称
Aromatase (CYP19A)抑制剂Screening试剂盒(Fluorometric)
参阅全部 Aromatase 试剂盒 -
检测方法
Fluorescent -
样品类型
Inhibitor compounds -
检测类型
Enzyme activity (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
The Aromatase Inhibitor Screening Kit (ab284522) (K984) enables rapid screening of drugs and other small molecules for compound-Aromatase interaction in a reliable, high-throughput fluorescence-based assay. The assay utilizes a fluorogenic substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 488/527 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K984 Aromatase (CYP19A) Inhibitor Screening Kit (Fluorometric). K984-100 is the same size as the 100 test size of ab284522.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate reader, Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests β-NADP Stock 1 vial 100X NADPH Generating System II 1 vial Aromatase Assay Buffer 1 x 100ml Aromatase Fluorescence Standard 1 x 50µl Aromatase Inhibitor 1 vial Aromatase Substrate 1 vial Recombinant Human Aromatase 2 vials -
研究领域
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功能
Catalyzes the formation of aromatic C18 estrogens from C19 androgens. -
组织特异性
Brain, placenta and gonads. -
疾病相关
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Membrane. - Information by UniProt
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别名
- ARO
- ARO1
- Aromatase
see all
图片
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Functional Studies - Aromatase standard curve
Aromatase Substrate metabolite fluorescence standard curve. One mole of fluorescence standard corresponds to the metabolism of one mole of Aromatase Substrate.
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Functional Studies - Reaction kinetics of recombinant human aromataseReaction kinetics of recombinant human aromatase enzyme at 37°C in the presence and absence of the indicated aromatase inhibitors. The solvent control reaction contained assay buffer with 0.2% acetonitrile.
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Functional studies - Dose-response curves for various aromatase ligandsDose-response curves for various aromatase ligands of differing structural and mechanistic classes. The potent competitive inhibitor letrozole, the mechanism-based irreversible inhibitor exemestane, the phytoestrogen-like synthetic flavonoid α-naphthoflavone and the antifungal ketoconazole (a competitive inhibitor of several CYP isoforms) are plotted in the graph. For dose-response curves, percent activity was calculated for each concentration of inhibitor by comparison to activity of reactions containing no inhibitor. For each inhibitor, IC50 values were derived by 4-parameter logistic curve fitting with each point representing the mean ± SEM of at least four replicates. Assays were performed according to the kit protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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