Aromatase (CYP19A) Activity Assay试剂盒(Fluorometric) (ab273306)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Lysate
概述
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产品名称
Aromatase (CYP19A) Activity Assay试剂盒(Fluorometric)
参阅全部 Aromatase 试剂盒 -
检测方法
Fluorescent -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Enzyme activity (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
Aromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions.
The assay utilizes a fluorogenic substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 488/527 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence.
A highly selective aromatase inhibitor is provided for determination of aromatase activity in heterogeneous biological samples, where other CYP isozymes may contribute to substrate metabolism. The inhibitor displays greater than 100-fold selectivity for aromatase over other enzymes, ensuring targeted inhibition. Aromatase specific activity is calculated by running parallel reactions in the presence and absence of the inhibitor and subtracting any residual activity detected with the inhibitor present.
The kit contains a complete set of reagents sufficient for 50 sets of paired reactions (in the presence and absence of inhibitor) and can detect a minimum of 25 nU aromatase activity.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K983 Aromatase (CYP19A) Activity Assay Kit (Fluorometric). K983-100 is the same size as the 100 test size of ab273306.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests β-NADP Stock 1 vial 100X NADPH Generating System II 1 vial Aromatase Assay Buffer 1 x 100ml Aromatase Fluorescence Standard 1 x 50µl Aromatase Inhibitor 1 vial Aromatase Substrate 1 vial Recombinant Human Aromatase 1 vial -
研究领域
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功能
Catalyzes the formation of aromatic C18 estrogens from C19 androgens. -
组织特异性
Brain, placenta and gonads. -
疾病相关
Defects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Membrane. - Information by UniProt
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别名
- ARO
- ARO1
- Aromatase
see all
图片
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Example standard curve
Standard curve of Aromatase Substrate metabolite fluorescence. One mole of fluorescence standard corresponds to the metabolism of one mole of Aromatase Substrate.
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Reaction KineticsReaction kinetics of fluorogenic substrate metabolism in donor-pooled human placental microsomes (0.5 mg/mL) at 37°C in the presence and absence of the competitive aromatase inhibitor letrozole (the no inhibitor condition contained assay buffer with 0.2% acetonitrile as a solvent control).
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Specific activity of aromatase in human placental microsome samplesSpecific activity of aromatase in human placental microsome samples (mean ± SEM of four independent replicates). Assays were performed according to the kit protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab273306 被引用在 2 文献中.
- Kim SY et al. Gulp1 deficiency augments bone mass in male mice by affecting osteoclasts due to elevated 17β-estradiol levels. J Cell Physiol 238:1006-1019 (2023). PubMed: 36870066
- Moutard L et al. Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice. Elife 12:N/A (2023). PubMed: 38095307