Apoptosis/ Necrosis Assay试剂盒(blue，red，green) (ab176750)
Key features and details
- Assay type: Cell-based
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
概述
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产品名称
Apoptosis/ Necrosis Assay试剂盒(blue，red，green)
参阅全部 Apoptosis/ Necrosis 试剂盒 -
样品类型
Adherent cells, Suspension cells -
检测类型
Cell-based -
检测时间
1h 00m -
产品概述
Apoptosis/ Necrosis Assay Kit (blue, red, green) (ab176750) is designed to simultaneously monitor apoptotic, necrotic and healthy cells.
The PS sensor used in this kit has red fluorescence (Ex/Em = 630/660 nm) upon binding to membrane PS.
Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents.
Loss of plasma membrane integrity, as demonstrated by the ability of the membrane-impermeable DNA Nuclear Green DCS1 dye (Ex/Em = 490/525 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage apoptosis and necrosis.
This apoptosis / necrosis assay also provides a live cell cytoplasm labeling dye CytoCalcein Violet 450 (Ex/Em = 405/450 nm) to label live cell cytoplasm.
This kit is optimized to simultaneously detect cell apoptosis (Red), apoptosis (red and/or green) and healthy cells (blue) with a flow cytometer or fluorescence microscope.
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说明
This product was previously called Apoptosis/ Necrosis Detection Kit.
Other apoptosis assays
For more apoptosis assays, review the apoptosis assay and apoptosis marker guide.
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平台
Flow cytometer, Fluorescence microscope
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Apopxin Deep Red Indicator 1 x 200µl Assay Buffer 1 x 50ml CytoCalcein Violet 450 1 vial Nuclear Green DCS1 Dye 1 x 100µl -
研究领域
图片
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Detection of binding activity of Apopxin Deep Red Indicator to phosphatidylserine in Jurkat cells using Apoptosis/Necrosis Detection Kit (blue, red, green) (ab176749)
The fluorescence image shows cells that are live (blue, stained by CytoCalcein Violet 450), apoptotic (red, stained by Apopxin Deep Red Indicator), and necrotic (green, indicated by Nuclear Green DCS1staining) in Jurkat cells induced by 1μM staurosporine for 3 hours. The fluorescence images of the cells were taken with a fluorescence microscope through the Violet, Cy5 and FITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Triple staining of staurosporine-induced cells.
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab176750 被引用在 16 文献中.
- Dinh CT et al. Single Fraction and Hypofractionated Radiation Cause Cochlear Damage, Hearing Loss, and Reduced Viability of Merlin-Deficient Schwann Cells. Cancers (Basel) 15:N/A (2023). PubMed: 37345155
- Varlamova EG et al. Pilot Study of Cytoprotective Mechanisms of Selenium Nanorods (SeNrs) under Ischemia-like Conditions on Cortical Astrocytes. Int J Mol Sci 24:N/A (2023). PubMed: 37569591
- Varlamova EG et al. Cerium Oxide Nanoparticles Protect Cortical Astrocytes from Oxygen-Glucose Deprivation through Activation of the Ca2+ Signaling System. Int J Mol Sci 24:N/A (2023). PubMed: 37762608
- Laval T et al. De novo synthesized polyunsaturated fatty acids operate as both host immunomodulators and nutrients for Mycobacterium tuberculosis. Elife 10:N/A (2021). PubMed: 34951591
- Clement S et al. Radiodynamic Therapy Using TAT Peptide-Targeted Verteporfin-Encapsulated PLGA Nanoparticles. Int J Mol Sci 22:N/A (2021). PubMed: 34204001