AB14150

Annexin V-Cy5 Apoptosis Staining / Detection试剂盒

Name: Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)| Abcam中文官网
Brand: Abcam Limited
SKU: AB14150
Price: 9000 CNY
Availability: InStock

Annexin V-Cy5 Apoptosis Staining / Detection Kit

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(27 Publications)

Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.

查看别名

ANX5, ENX2, PP4, ANXA5, Annexin A5, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, Placental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, CPB-I, PAP-I, VAC-alpha

1 Images

Flow Cytometry - Annexin V-Cy5 Apoptosis Staining / Detection Kit (AB14150)

Flow Cyt

PubMed

Flow Cytometry - Annexin V-Cy5 Apoptosis Staining / Detection Kit (AB14150)

Impact of different Rep constructs on DNA damage response and apoptosis.

Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.

Image from Seyffert M et al., PLoS One 12(1), fig 3d. Doi: 10.1371/journal.pone.0170908. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

关键信息

样品类型

Suspension cells, Adherent cells

检测类型

Direct

检测时间

10m

检测平台

Flow cytometer, Fluorescence microscope

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产品详情

Fluorescence can be detected at Ex/Em = 649/670 nm.

Analysis is by flow cytometry or fluorescence microscopy.

The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent ab14147.

Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine. For more apoptosis assays, review the full set of , or the .

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

The protein expressed by gene ANXA5 is an anticoagulant protein that indirectly inhibits the thromboplastin-specific complex, a component involved in the blood coagulation cascade. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ANXA5

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文献 (27)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111404 PubMed39687021

2024

A combined AI and cell biology approach surfaces targets and mechanistically distinct Inflammasome inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Chen,Tempest Plott,Michael Wiest,Will Van Trump,Ben Komalo,Dat Nguyen,Charlie Marsh,Jarred Heinrich,Colin J Fuller,Lauren Nicolaisen,Elisa Cambronero,An Nguyen,Christian Elabd,Francesco Rubbo,Rachel DeVay Jacobson

Annals of translational medicine 9:1641 PubMed34988150

2022

assessment of an engineered tBID-based safety switch system in human T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamiao Lu,Patrick Collins,Ki Jeong Lee,Chi-Ming Li,Songli Wang

EMBO molecular medicine 14:e14511 PubMed34779136

2021

LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Pin Li,Qin Li,Nilima Biswas,Hong Xin,Tanja Diemer,Lixian Liu,Lorena Perez Gutierrez,Giovanni Paternostro,Carlo Piermarocchi,Sergii Domanskyi,Ruikang K Wang,Napoleone Ferrara

Proceedings of the National Academy of Sciences of the United States of America 117:14694-14702 PubMed32554491

2020

DNA-based fluorescent probes of NOS2 activity in live brains.

Applications

Unspecified application

Species

Unspecified reactive species

Aneesh T Veetil,Junyi Zou,Katharine W Henderson,Maulik S Jani,Shabana M Shaik,Sangram S Sisodia,Melina E Hale,Yamuna Krishnan

Nature communications 11:685 PubMed32019925

2020

A central role of IKK2 and TPL2 in JNK activation and viral B-cell transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Voigt,Kai R Sterz,Fabian Giehler,Anne-Wiebe Mohr,Joanna B Wilson,Andreas Moosmann,Arnd Kieser

Cell reports 27:3422-3432.e4 PubMed31216465

2019

Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Lynnette Marcar,Kankana Bardhan,Liliana Gheorghiu,Patrick Dinkelborg,Heike Pfäffle,Qi Liu,Meng Wang,Zofia Piotrowska,Lecia V Sequist,Kerstin Borgmann,Jeffrey E Settleman,Jeffrey A Engelman,Aaron N Hata,Henning Willers

Oncology reports 41:989-998 PubMed30431119

2018

Glutathione S‑transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Zou,Xiaolei Hu,Bangtian Xu,Tingting Tong,Yixian Jing,Lei Xi,Wei Zhou,Jinpeng Lu,Xin Wang,Xiaolan Yang,Fei Liao

Journal of cell science 131: PubMed29930080

2018

Transbilayer phospholipid movement facilitates the translocation of annexin across membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Stewart,Avraham Ashkenazi,Athena Williamson,David C Rubinsztein,Kevin Moreau

American journal of physiology. Renal physiology 315:F130-F137 PubMed29561184

2018

Functional transport of organic anions and cations in the murine mesonephros.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie L Lawrence,James R Smith,Jamie A Davies

Stem cells translational medicine 7:115-124 PubMed29119710

2017

Role of SDF-1:CXCR4 in Impaired Post-Myocardial Infarction Cardiac Repair in Diabetes.

Applications

FuncS

Species

Mouse

Maritza E Mayorga,Matthew Kiedrowski,Patricia McCallinhart,Farhad Forudi,Jeremiah Ockunzzi,Kristal Weber,William Chilian,Marc S Penn,Feng Dong

View all publications

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Annexin V-Cy5 Apoptosis Staining / Detection试剂盒

Annexin V-Cy5 Apoptosis Staining / Detection Kit

Flow Cytometry - Annexin V-Cy5 Apoptosis Staining / Detection Kit (AB14150)

关键信息

样品类型

检测类型

检测时间

检测平台

产品详情