AMP Assay试剂盒(Colorimetric) (ab273275)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
AMP Assay试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Quantitative -
实验步骤
Multiple steps standard assay -
产品概述
AMP Assay Kit (Colorimetric) (ab273275) provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples.
The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K229 AMP Colorimetric Assay Kit. K229-100 is the same size as the 100 test size of ab273275.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests AMP 1 x 200µl Assay Buffer XV 1 x 25ml AMP Substrate Mix 1 vial Developer V 1 vial Development Enzyme Mix III 1 x 200µl OxiRed Probe 1 x 200µl
图片
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataMeasurement of AMP in different cell lysates: Jurkat (20 µg), HeLa (30 µg) and HEK293 (60 μg).
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab273275 被引用在 3 文献中.
- Domínguez-Zorita S et al. IF1 ablation prevents ATP synthase oligomerization, enhances mitochondrial ATP turnover and promotes an adenosine-mediated pro-inflammatory phenotype. Cell Death Dis 14:413 (2023). PubMed: 37433784
- Zhang YL et al. Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway. Oxid Med Cell Longev 2022:6196173 (2022). PubMed: 35602095
- Yang W et al. Rheb mediates neuronal-activity-induced mitochondrial energetics through mTORC1-independent PDH activation. Dev Cell 56:811-825.e6 (2021). PubMed: 33725483