alpha-L-Fucosidase Assay试剂盒(ab272525)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
概述
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产品名称
alpha-L-Fucosidase Assay试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Plasma, Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
范围
1 mU/ml - 100 mU/ml -
产品概述
alpha-L-Fucosidase Assay Kit (ab272525) is a non-radioactive, colorimetric assay based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.
High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L for a 20 minute reaction.
Homogeneous and simple procedure. Simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.
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经测试应用
适用于: Functional Studiesmore details -
平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 100 tests Standard (12.5 mM Nitrophenol) 1 x 1ml Stop Reagent 1 x 12ml Substrate Buffer 1 x 10ml -
研究领域
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细胞定位
FUCA1: Lysosome. FUCA2: Secreted.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272525于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example Standard Curve.
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Activity Titration CurveExample activity titration curve.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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