alpha-Glucosidase抑制剂Screening试剂盒(Colorimetric) (ab284520)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Inhibitor compounds
概述
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产品名称
alpha-Glucosidase抑制剂Screening试剂盒(Colorimetric)
参阅全部 GAA 试剂盒 -
检测方法
Colorimetric -
样品类型
Inhibitor compounds -
检测类型
Enzyme activity (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
The α-Glucosidase Inhibitor Screening Kit (ab284520) (K938) can be used to screen potential inhibitors of this enzyme. It utilizes the ability of an active α-Glucosidase to cleave a synthetic substrate thus, releasing a chromophore (OD: 410 nm). In the presence of an α-Glucosidase specific inhibitor, the enzymatic activity is greatly reduced which is detected by a decrease of absorbance readings.
The assay kit provides a rapid, simple and reliable test for high-throughput screening of α-Glucosidase inhibitors.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K938 a-Glucosidase Inhibitor Screening Kit (Colorimetric). K938-100 is the same size as the 100 test size of ab284520.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests α-Glucosidase Assay Buffer 1 x 25ml α-Glucosidase Positive Control 1 vial Acarbose 1 x 140µl α-Glucosidase Substrate Mix 1 x 300µl -
研究领域
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功能
Essential for the degradation of glygogen to glucose in lysosomes. -
疾病相关
Defects in GAA are the cause of glycogen storage disease type 2 (GSD2) [MIM:232300]; also called acid alpha-glucosidase (GAA) deficiency or acid maltase deficiency (AMD). GSD2 is a metabolic disorder with a broad clinical spectrum. The severe infantile form, or Pompe disease, presents at birth with massive accumulation of glycogen in muscle, heart and liver. Cardiomyopathy and muscular hypotonia are the cardinal features of this form whose life expectancy is less than two years. The juvenile and adult forms present as limb-girdle muscular dystrophy beginning in the lower limbs. Final outcome depends on respiratory muscle failure. Patients with the adult form can be free of clinical symptoms for most of their life but finally develop a slowly progressive myopathy. -
序列相似性
Belongs to the glycosyl hydrolase 31 family.
Contains 1 P-type (trefoil) domain. -
翻译后修饰
The different forms of acid glucosidase are obtained by proteolytic processing.
Phosphorylation of mannose residues ensures efficient transport of the enzyme to the lysosomes via the mannose 6-phosphate receptor. -
细胞定位
Lysosome. Lysosome membrane. - Information by UniProt
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别名
- 70 kDa lysosomal alpha-glucosidase
- Acid alpha glucosidase
- Acid maltase
see all
图片
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Inhibition of alpa-Glucosidase activity by Acarbose
α-Glucosidase activity inhibition by Acarbose. IC50 of Acarbose was calculated to be 0.74 ± 0.15 mM. Assay was carried out following the kit protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab284520 被引用在 2 文献中.
- Jung J et al. The Immune-Stimulating and Anti-Diabetic Effects of Allium hookeri Leaves Grown in a Plant Factory with Artificial Lights in Immunosuppressed Obese C57BL/6 Mice. Pharmaceuticals (Basel) 17:N/A (2024). PubMed: 38256924
- Ahamad Tarmizi AA et al. Phytofabrication of Selenium Nanoparticles with Moringa oleifera (MO-SeNPs) and Exploring Its Antioxidant and Antidiabetic Potential. Molecules 28:N/A (2023). PubMed: 37513196