Aldose Reductase Activity试剂盒(Colorimetric) (ab273276)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 100 µU
概述
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产品名称
Aldose Reductase Activity试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Tissue, Adherent cells, Suspension cells -
检测类型
Enzyme activity (quantitative) -
灵敏度
100 µU -
实验步骤
Multiple steps standard assay -
产品概述
Aldose Reductase Activity Assay Kit (Colorimetric) (ab273276) utilizes the ability of Aldose Reductase (AR) to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The kit can detect as low as 0.1 mU. The assay kit is simple and can be used in a high-throughput format.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K369 Aldose Reductase Activity Kit (Colorimetric). K369-100 is the same size as the 100 test size of ab273276.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Active Aldose Reductase 1 vial AR Assay Buffer 1 x 35ml AR Substrate 1 x 1ml DTT I 1 x 0.4ml NADPH V 1 vial 96-Well UV Transparent Plate 1 unit -
研究领域
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功能
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. -
组织特异性
Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. -
序列相似性
Belongs to the aldo/keto reductase family. -
细胞定位
Cytoplasm. - Information by UniProt
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别名
- ADR
- AKR1B 1
- Akr1b1
see all
图片
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NADPH standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Purified Aldose Reductase activityPurified Aldose Reductase activity.
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Aldose Reductase Activity in Tissue SamplesAldose Reductase specific activity was calculated in Rat Kidney tissue lysates (20 µg protein) and HepG2 cell lysates (110 µg protein).
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab273276 被引用在 2 文献中.
- Radenkovic S et al. Tracer metabolomics reveals the role of aldose reductase in glycosylation. Cell Rep Med 4:101056 (2023). PubMed: 37257447
- Cao Y et al. Propofol suppresses cell proliferation in gastric cancer cells through NRF2-mediated polyol pathway. Clin Exp Pharmacol Physiol 49:264-274 (2022). PubMed: 34570396