Advanced Glycation End Products (AGEs) Assay试剂盒(ab273298)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Serum
概述
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产品名称
Advanced Glycation End Products (AGEs) Assay试剂盒
参阅全部 AGE 试剂盒 -
检测方法
Fluorescent -
样品类型
Serum -
检测类型
Semi-quantitative -
实验步骤
Multiple steps standard assay -
产品概述
Advanced Glycation End Products (AGEs) Activity Assay Kit (ab273298) can be used for the semi-quantitative estimation of fluorescent AGEs levels in Biological Fluids. The assay is based on the characteristic fluorescence (Ex/Em= 360/460 nm), a characteristic that is shared by almost all AGEs. The proprietary composition of the Assay Buffer specifically distinguishes between AGEs and non-oxidized proteins. The one-step assay uses oxidized Bovine Serum Albumin (AGE-BSA) as a Positive Control. BSA is used as Background Control and its fluorescence under assay conditions is defined as 1 relative fluorescence intensity in arbitrary units (AU or RFUSample/ RFUBackground).
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K929 Advanced Glycation End Products (AGEs) Assay Kit. K929-100 is the same size as the 100 test size of ab273298.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests AGE Positive Control Protein 1 x 500µl BSA Standard I 1 x 500µl Wash Buffer II 1 x 50ml -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
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相关性
The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure. -
细胞定位
Cell Membrane and Secreted -
别名
- Advanced glycation end products
- AGEs
图片
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AGE and BSA standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
AGEs-Positive (AGE-BSA) fluorescence versus BSA. The assay can distinguish both species (AGEs and non-oxidized proteins).
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AGE activity in serum samplesAGEs amount in Pooled Human Serum from healthy donors (4 µg serum protein), Human Diabetic Serum (4 µg serum protein) and Human Urine from healthy donor.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab273298 被引用在 2 文献中.
- Huo S et al. ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury. Int J Mol Sci 24:N/A (2023). PubMed: 36675183
- Heng YY et al. Sodium tanshinone IIA sulfonate ameliorates neointima by protecting endothelial progenitor cells in diabetic mice. BMC Cardiovasc Disord 23:446 (2023). PubMed: 37697234