JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB323608

Adenylate Cyclase Activity Assay

Adenylate Cyclase Activity Assay

Be the first to review this product! Submit a review

|

(0 Publication)

The Adenylate Cyclase Activity Assay Kit (ab323608) is a simple, sensitive kit that measures the conversion of ATP to cAMP and pyrophosphate using a propriety enzymatic detection mix and a chromogenic probe to generate a stable chromophore that can be quantified by absorbance.
4 Images
Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
  • FuncS

Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Adenylate Cyclase Activity Assay Kit reaction pathway

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
  • FuncS

Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Example standard curve (0-10 nmol) used to determine the activity of adenylate cyclase. The change in absorbance over a time range for a sample is used to determine the nmol of PPi generated due to adenylate cyclase activity in a defined time frame. ΔAbsorbance must be within the range of the standard curve.

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)
  • FuncS

Supplier Data

Functional Studies - Adenylate Cyclase Activity Assay (AB323608)

Example midrange positive control data. The linear range for this positive control occurs between 5 and 18 minutes. Prior to five minutes, the reaction is coming to temperature and is experiencing a lag as the enzyme ramps up, after 18 minutes the substrate is becoming depleted and activity is slowing. Choosing a time interval within the linear range for each sample is critical to accurately quantify adenylate cyclase activity.

Biochemical assay - Adenylate Cyclase Activity Assay (AB323608)
  • Biochemical assay

Supplier Data

Biochemical assay - Adenylate Cyclase Activity Assay (AB323608)

Adenylate Cyclase activity in cell and tissue lysates. Samples were lysed according to the sample preparation protocol and 4 µl of each sample was tested. Specific activity was calculated from sample concentration determined using a Bradford assay.

关键信息

检测方法

Colorimetric

样品类型

Tissue Lysate, Cell Lysate

检测类型

Enzyme activity

Quantitative
灵敏度

< 0.02 mU

范围

0.02 - 1 mU

检测时间

1h 15m

检测平台

Microplate

style
left-column

搭配使用产品

Assay Kits

AB138880

cAMP Assay Kit (Competitive ELISA, Fluorometric)

ELISA - cAMP Assay Kit (Competitive ELISA, Fluorometric) (AB138880)

  • 0
  • 0
Assay Kits

AB211175

ROCK Activity Assay Kit

Functional Studies - ROCK Activity Assay Kit (AB211175)

  • 0
  • 0
Assay Kits

AB289834

Mycoplasma PCR Detection Kit

Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)

  • 0
  • 0
Assay Kits

AB290713

cAMP Assay Kit (Competitive ELISA)

Competitive ELISA - cAMP Assay Kit (Competitive ELISA) (AB290713)

  • 0
  • 0
style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biochemical assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

The Adenylate Cyclase Activity assay kit is a simple, sensitive kit that measures the conversion of ATP to cAMP and pyrophosphate using a propriety enzymatic detection mix and a chromogenic probe to generate a stable chromophore that can be quantified by absorbance.

In the first step of this enzymatic assay, Adenylyl Cyclase (Adenylate Cyclase) converts ATP into cAMP, releasing inorganic pyrophosphate (PPi). The PPi released is detected via a series of enzymatic reactions which utilize a propriety enzyme mix and a chromogenic probe, generating a stable chromophore that can be quantified by absorbance at 570 nm.

First functional test on catalogue, others are ELISA based and either only measure cAMP levels or AC protein concentration.

style
left-column

规格

{ "values": { "1x100Tests": { "sellingSize": "1 x 100 Tests", "publicAssetCode":"ab323608-1x100Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Pyrophosphate Standard", "number":"AB323608-CMP08", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB323608-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB323608-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Enzyme Mix X", "number":"AB323608-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"AC Substrate Mix", "number":"AB323608-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 15", "number":"AB323608-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"AC Positive Control", "number":"AB323608-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"ATP IV", "number":"AB323608-CMP02", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

See full target information Adenylate Cyclase
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com