Adenosine Assay试剂盒(Fluorometric)

产品详情

How the assay works

In Adenosine Assay Kit (Fluorometric), adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the adenosine probe, leading to the formation of a fluorescent product. The fluorescent product can be detected at Ex/Em = 535/587 nm, and its intensity is proportional to the amount of adenosine in the sample.

Adenosine assay protocol summary:

- Add samples and standards to wells
- Add reaction mix
- Incubate for 60 min at 37°C
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm)

How other researchers are using

Adenosine Assay Kit (Fluorometric) has been used in a variety of sample type including:
- Mouse immortalized microglial cell line BV-2 ¹
- Mouse bronchoalveolar lavage (BAL) fluid ²
- Mouse Splenic CD4+CD25+ Tregs or CD4+CD25- T cells ³

References:

1-Tscherrig V al. 2023
2 Kelestemur T et al. 2023
3-Kong L et al. 2023

This product is manufactured by BioVision, an Abcam company and was previously called K327 Adenosine Assay Kit (Fluorometric). K327-100 is the same size as the 100 test size of ab211094.

Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.