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Acid Phosphatase Assay试剂盒(Colorimetric) (ab83367)

Submit a review Q&A (2)References (15)
Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 1 hr 10 min
  • Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.001 mM

概述

  • 产品名称

    Acid Phosphatase Assay试剂盒(Colorimetric)
    参阅全部 Acid phosphatase 试剂盒

  • 检测方法

    Colorimetric

  • 样品类型

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media

  • 检测类型

    Enzyme activity

  • 灵敏度

    > 0.001 mM

  • 范围

    0.001 mM - 1 mM

  • 检测时间

    1h 10m

  • 产品概述

    Acid Phosphatase Assay Kit ab83367 is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure acid phosphatase activity in serum and other samples.


    The acid phosphatase assay protocol uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (absorbance max 405 nm) when dephosphorylated by acid phosphatase.


    Acid phosphatase assay protocol summary:
    - add samples and standards to wells
    - add pNPP to samples
    - add acid phosphatase enzyme to standards
    - incubate for 60 min
    - add stop solution
    - analyze with microplate reader

  • 说明

    This product is manufactured by BioVision, an Abcam company and was previously called K411 Acid Phosphatase Activity Colorimetric Assay Kit. K411-500 is the same size as the 500 test size of ab83367.

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease.

    pNPP Assays

    pNPP assays are a class of phosphatase assays using p-nitrophenyl phosphate (pNPP) as a phosphatase substrate. They can be used to measure alkaline phosphatase, neutral phosphatase, and acid phosphatase activity. These different types of phosphatases are active respectively in alkaline, neutral, and acid assay buffers. pNPP assays include Alkaline Phosphatase Assay Kit ab83369 and Acid Phosphatase Assay Kit ab83367.

  • 平台

    Microplate reader

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 500 tests
    AP Assay Buffer 1 x 100ml
    AP Enzyme 1 vial
    pNPP Substrate 1 vial
    Stop Solution I 1 x 10ml

  • 研究领域

  • 相关性

    Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.

  • 细胞定位

    ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein.

  • 别名

    • Acid phosphatase 1 soluble
    • Acid phosphatase 2 lysosomal
    • Acid phosphatase 5 tartrate resistant
    • Acid phosphatase of erythrocyte
    • Acid phosphatase prostate
    • ACP1
    • ACP2
    • ACP3
    • ACP5
    • ACPP
    • Adipocyte acid phosphatase
    • HAAP
    • LAP
    • LMW PTP
    • LMW PTPase
    • Low molecular weight cytosolic acid phosphatase
    • Low molecular weight phosphotyrosine protein phosphatase
    • Lysosomal acid phosphatase
    • PAP
    • pnpp
    • Prostatic acid phosphotase
    • Red cell acid phosphatase 1
    • Tartrate resistant acid ATPase
    • Tartrate resistant acid phosphatase 5
    • Tartrate resistant acid phosphatase type 5
    • TR AP
    • TRAP
    • TrATPase
    see all

图片

  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    AP Enzyme samples 40 minute assay
  • Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    Functional Studies - Acid Phosphatase Colorimetric Assay Kit (ab83367)
    p-Nitrophenol (pNP) standard curve

文献 (15)

发表研究结果有使用 ab83367？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab83367 被引用在 15 文献中.

  • Yang H  et al. MEK1 drives oncogenic signaling and interacts with PARP1 for genomic and metabolic homeostasis in malignant pleural mesothelioma. Cell Death Discov 9:55 (2023). PubMed: 36765038
  • Pastwinska J  et al. Hypoxia modulates human mast cell adhesion to hyaluronic acid. Immunol Res 70:152-160 (2022). PubMed: 34791576
  • Zheng ZG  et al. Discovery of a potent SCAP degrader that ameliorates HFD-induced obesity, hyperlipidemia and insulin resistance via an autophagy-independent lysosomal pathway. Autophagy 17:1592-1613 (2021). PubMed: 32432943
  • Yang H  et al. NF2 and Canonical Hippo-YAP Pathway Define Distinct Tumor Subsets Characterized by Different Immune Deficiency and Treatment Implications in Human Pleural Mesothelioma. Cancers (Basel) 13:N/A (2021). PubMed: 33805359
  • Yang H  et al. Pharmaco-transcriptomic correlation analysis reveals novel responsive signatures to HDAC inhibitors and identifies Dasatinib as a synergistic interactor in small-cell lung cancer. EBioMedicine 69:103457 (2021). PubMed: 34224975
View all Publications for this product

客户评价及客户问答

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Question

I am enquiring as to whether this kit that can be used as a tartrate-resistant acid phosphatase quantification assay. The nature of my samples are hard to visualise microscopically so a TRAP stain will not work. I haven't kept the supernatant either so TRAP quantification in the media is not possible. Can this assay be used to quantify the amount of TRAP in the cell lysate, do you think this would work?

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Answer



If the TRAP is able to perform the same functions (phosphorylation of the substrates) as any other AP, then yes, this kit could be used. However, we cannot guarantee that it will definitely work as we have never tried it to quantify TRAP.

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Question

Could you please provide the buffer constituents details with information on molar, pH, presence/absence of protease inhibitor, etc?

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Answer

Thank you for your enquiry.

Unfortunately, most of the information you asked for is proprietary info. However, I can confirm that the assay buffer does not contain protease inhibitors and its pH is 5.0.

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