5’-Nucleotidase (CD73) Activity Assay试剂盒(Colorimetric) (ab235945)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
概述
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产品名称
5’-Nucleotidase (CD73) Activity Assay试剂盒(Colorimetric)
参阅全部 CD73 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell Lysate, Tissue Lysate -
检测类型
Quantitative -
范围
2 nmol/well - 15 nmol/well -
种属反应性
预测可用于: Mammals -
产品概述
5’-Nucleotidase (CD73) Activity Assay Kit (Colorimetric) (ab235945) is a simple two-step end-point assay for the measurement of 5’-Nucleotidase activity in various tissues/cells. This assay relies on the Berthelot’s test for quantification of ammonia.
In this assay, the action of 5’-nucleotidase on the substrate generates a product, which releases ammonia in presence of the converter. Developer I and II are then used to quantify the released ammonia through increase in absorbance at 670 nm.
This assay can detect as low as 0.2 mU of 5’-NT. Since non-specific enzymes like alkaline phosphatase can give a positive signal in this assay, 5’-NT inhibitor may be used to completely inhibit 5’-nucleotidase and distinguish from the signal from non-specific enzymes.
The assay kit also includes 5’- Nucleotidase (5’-NT) enzyme for use as positive control.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K992 5’-Nucleotidase Activity Assay Kit (Colorimetric). K992-100 is the same size as the 100 test size of ab235945.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 5’-NT Substrate 1 vial 5'-NT Buffer 1 x 25ml 5'NT Convertor 1 vial 5'-NT Inhibitor 1 x 250µl 5'-NT Positive Control 1 vial Ammonium Standard II 1 x 100µl EDTA Solution 1 x 500µl Reagent II 1 x 8ml Reagent III 1 x 4ml -
研究领域
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功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
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别名
- 5' NT
- 5' nucleotidase (CD73)
- 5' nucleotidase precursor
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab235945 被引用在 1 文献中.
- Li H et al. Blockade pf CD73/adenosine axis improves the therapeutic efficacy of docetaxel in epithelial ovarian cancer. Arch Gynecol Obstet 299:1737-1746 (2019). PubMed: 30941556