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3' to 5' Exonuclease Activity Assay试剂盒(Fluorometric) (ab273269)

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3' to 5' Exonuclease Kinetics
  • 3' to 5' Exonuclease Activity in different Sample types
  • 3' to 5' Exonuclease Activity
  • Fluorescence Standard Curve

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate
  • Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Extracts

概述

  • 产品名称

    3' to 5' Exonuclease Activity Assay试剂盒(Fluorometric)

  • 检测方法

    Colorimetric/Fluorometric

  • 样品类型

    Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts

  • 检测类型

    Enzyme activity (quantitative)

  • 实验步骤

    Multiple steps standard assay

  • 产品概述

    3' to 5' Exonuclease Activity Assay Kit (Fluorometric) (ab273269) provides a quick and easy method for monitoring 3’ exonuclease activities in a wide variety of samples. In this assay, exonuclease digests the provided DNA probe, producing a strong fluorescent signal (Ex/Em = 304/369nm). The kit is simple, sensitive, high-throughput adaptable and can detect as low as 0.2 µU of exonuclease activity.

  • 说明

    This product is manufactured by BioVision, an Abcam company and was previously called K175 3’ to 5’ Exonuclease Activity Assay (Fluorometric). K175-100 is the same size as the 100 test size of ab273269.

    The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

  • 平台

    Microplate

性能

  • 存放说明

    Store at -20°C. Please refer to protocols.
  • 组件 100 tests
    Exonuclease Assay Buffer 1 x 25ml
    Exonuclease Positive Control 1 vial
    Exonuclease Probe 1 vial
    Fluorescence Standard II 1 x 100µl
    96-Well Half Area Plate (with lid) 1 unit

图片

  • 3' to 5' Exonuclease Kinetics
    3' to 5' Exonuclease Kinetics

    Kinetic curves of rat liver extract (40.6 μg), HeLa cell lysate(12.2 μg) and E.coli (0.642 μg).

  • 3' to 5' Exonuclease Activity in different Sample types
    3' to 5' Exonuclease Activity in different Sample types

    Specific activity from rat liver extract (0.0206 μU/μg). HeLa cell lysate (0.0841 μU/μg) and E.coli crude extract (0.9071 μU/μg). Assays performed according to the kit protocol.  

  • 3' to 5' Exonuclease Activity
    3' to 5' Exonuclease Activity

    Specific activity of  the probe to 3’ to 5’ Exonuclease (Exonucleae III) in contrast to DNAase I. Same units of proteins were added as defined by the commercially available values.

  • Fluorescence Standard Curve
    Fluorescence Standard Curve

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

    Fluorescence Standard Curve.

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