1,5-Anhydroglucitol Assay试剂盒(Colorimetric) (ab284531)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Plasma, Saliva, Serum
概述
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产品名称
1,5-Anhydroglucitol Assay试剂盒(Colorimetric) -
检测方法
Colorimetric -
样品类型
Saliva, Serum, Plasma -
检测类型
Enzyme activity (quantitative) -
实验步骤
Multiple steps standard assay -
产品概述
1,5-Anhydroglucitol Assay Kit (Colorimetric) (ab284531) (K2029) is a high-throughput adaptable microplate-based assay that enables rapid quantification of 1,5-AG levels in biological fluids. The assay consists of two steps: in the first step, the sample is pretreated to remove endogenous glucose that may interfere with the 1,5-AG detection and 1,5-AG is converted to 1,5-AG-6-phosphate. In the second step, 1,5-AG-6- phosphate is enzymatically metabolized, generating NADPH, which subsequently reacts with a colorless probe to form a strongly colored product measured by absorbance at OD = 460 nm. The absorbance is proportional to the concentration of 1,5-AG present in the samples.
The assay is not affected by galactose, fructose or other physiologically common sugars and has a limit of detection of 0.11 nmoles of 1,5- AG per well, with a reliable linear range from 0.2 – 5 nmole per well (corresponding to a 20-500 μM concentration in a 10 μl sample volume).
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K2029 1,5-Anhydroglucitol Assay Kit (Colorimetric). K2029-100 is the same size as the 100 test size of ab284531.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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经测试应用
适用于: Enzyme activity assaymore details -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Please refer to protocols. -
组件 100 tests 1,5-AG Standard 1 vial 96-Well Half Area Plate 1 unit Detection Buffer 1 x 10ml Detection Enzyme Mix 1 vial NADP Detection Probe 1 vial Pretreatment Cofactor Mix 1 vial Pretreatment Enzyme Mix 1 vial Sample Pretreatment Buffer 1 x 10ml
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab284531于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Enzyme activity assay |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Enzyme activity assay
Use at an assay dependent concentration. |
图片
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1,5-Anhydroglucitol Assay Kit (Colorimetric)
1,5-Anhydroglucitol (1,5-AG) Standard Curve. 1,5-AG concentration is directly proportional to the absorbance measured at 460 nm. For demonstrative purposes only.
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1,5-Anhydroglucitol Assay Kit (Colorimetric)Estimation of 1,5-AG in two different single-donor “off-the-clot” human serum samples (10 μl), each spiked with 50 μM 1,5- AG. Mean 1,5-AG concentrations detected in the samples were 149.4 ± 14.2 μM and 66.1 ± 7.2 μM, respectively (mean spike recovery rates for the two samples were 107.9% and 101.6%, respectively). Data are mean ± SD of 4-5 replicates, assayed according to the kit protocol. For demonstrative purposes only.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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