Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (AB109884)

Immunocytochemistry/Immunofluorescence analysis of NCCIT(human pluripotent embryonal carcinoma) cells labelling Nanog with purified ab109250 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (AB109884)

This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.

This image is courtesy of an anonymous collaborator.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (AB109884)

NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.

This image is courtesy of an anonymous collaborator.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Embryonic Stem Cell Marker Panel (Human: Oct4, Nanog, Tra-1-60 (R), SOX2, SSEA4) (AB109884)

ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C.

This image is courtesy of an anonymous collaborator.