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AB169809

人Th1/Th2/Th17抗体阵列-膜(34 targets)

Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets)

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(2 Publications)

ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines.

查看别名

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

3 Images
ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • ELISA

Unknown

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

PBMC.

Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • ELISA

Unknown

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

Human serum and plasma (EDTA) from a pooled donor (n=50) sample was diluted to 25% and assayed using ab169809. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)
  • Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

关键信息

反应种属

Human

靶标

TNFRSF8

target

应用

Multiplex Protein Detection

applications

样品类型

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

ab169809 is for the simultaneous detection of 34 Human Th1, Th2, and Th17 cytokines. Suitable for all liquid sample types.

Targets: CD30, CD40 Ligand, CD40, G-CSF, GITR, GM-CSF, IFN-gamma, IL-1 beta, IL-1 sRI, IL-1 sRII, IL-2, IL-4, IL-5, IL-6, IL-6 R, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17, IL-17F, IL-17 R, IL-21, IL-21R, IL-22, IL-23 (p19), IL-28A, MIP-3 alpha, spg130, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TRANCE.

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8

其他靶点

CD40,CD40LG,IL10,IL12B,IL12A,IL13,IL17RA,IL17A,IL17F,IL2,IL21,IL21R,CSF3,IL22,IFNL2,IL4,IL5,IL6,IL6R,CCL20,TGFB1,TGFB3,TNFRSF18,TNF,LTA,TNFSF11,CSF2,IL6ST,IFNG,IL1B,IL1R1,IL1R2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35682603

2022

Human Omental Mesothelial Cells Impart an Immunomodulatory Landscape Impeding B- and T-Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Benoit R Gauthier,Diana Rubio-Contreras,Juan Carlos Gómez-Rosado,Luis Cristobal Capitán-Morales,Abdelkrim Hmadcha,Bernat Soria,Christian Claude Lachaud

International journal of molecular sciences 23: PubMed35328606

2022

Reduced Expression of PD-1 in Circulating CD4+ and CD8+ Tregs Is an Early Feature of RRMS.

Applications

Unspecified application

Species

Unspecified reactive species

Maja Machcińska,Magdalena Kierasińska,Martyna Michniowska,Marta Maruszewska-Cheruiyot,Ludmiła Szewczak,Rafał Rola,Anna Karlińska,Michael Stear,Katarzyna Donskow-Łysoniewska
View all publications
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websiteProtocolBooklet
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搭配使用产品

Reagents, Controls & Accessories

AB270055

Protease Inhibitor Cocktail EDTA Free (100X)

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Multiplex Kits

AB133997

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

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  • 0
Multiplex Kits

AB133998

Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets) (AB133998)

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  • 0
Multiplex Kits

AB134003

Human Inflammation Antibody Array - Membrane (40 Targets)

Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (40 Targets) (AB134003)

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  • 0
Multiplex Kits

AB133996

Cytokine Array - Human Cytokine Antibody Array (Membrane, 23 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 23 Targets) (AB133996)

  • 0
  • 0

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