JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB197457

人Th1/Th2/Th17抗体阵列(20 Targets) - Quantitative

Human Th1/Th2/Th17 Antibody Array (20 Targets) - Quantitative

Be the first to review this product! Submit a review

|

(0 Publication)

ab197457 can be used for quantitative measurement of 20 Human Cytokines.

查看别名

LARC, MIP3A, SCYA20, CCL20, C-C motif chemokine 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20, MIP-3-alpha

2 Images
Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array (20 Targets) - Quantitative (AB197457)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array (20 Targets) - Quantitative (AB197457)

Array Map for Abcam Human Th1/Th2/Th17 Antibody Array (20 Targets)- Quantitative (ab197457)

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array (20 Targets) - Quantitative (AB197457)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Th1/Th2/Th17 Antibody Array (20 Targets) - Quantitative (AB197457)

Standard Curve obtained with Abcam Human Th1/Th2/Th17 Antibody Array (20 Targets)- Quantitative (ab197457)

关键信息

检测类型

Quantitative

反应种属

Human

靶标

CCL20

target

检测方法

Fluorescent

检测平台

Multiplex

样品类型

Tissue Lysate, Plasma, Cell culture media, Serum, Other biological fluids, Cell Lysate

style
left-column

产品详情

ab197457 can be used for quantitative measurement of 20 Human Cytokines. Suitable for serum, plasma, cell culture media, other body fluid, cell and tissue lysates.
Target names: GM-CSF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17, IL-17F, IL-21, IL-22, IL-23, IL-28, IFNγ, MIP-3α, TGFβ1, TNFα, TNFβ.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. The standard is affinity purified.

style
left-column

规格

{ "values": { "22Test": { "sellingSize": "22 Test", "publicAssetCode":"ab197457-22Test", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Adhesive Film", "number":"AB197457-CMP09" }, { "size":"1 x 1 Vial", "name":"Human Th1/Th2/Th17 Array (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197457-CMP05" }, { "size":"2 x 1 Slide", "name":"Human Th1/Th2/Th17 Array (20 T) Glass Slide", "number":"AB197457-CMP04" }, { "size":"2 x 25 µL", "name":"Human Th1/Th2/Th17 Array (20 T) Biotinylated Antibody Cocktail", "number":"AB197457-CMP03" }, { "size":"3 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197457-CMP06" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197457-CMP01" }, { "size":"2 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197457-CMP08" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197457-CMP07" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197457-CMP02" } ] }, "8Test": { "sellingSize": "8 Test", "publicAssetCode":"ab197457-8Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Adhesive Film", "number":"AB197457-CMP09" }, { "size":"1 x 1 Vial", "name":"Human Th1/Th2/Th17 Array (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197457-CMP05" }, { "size":"1 x 15 mL", "name":"Sample Diluent", "number":"AB197457-CMP01" }, { "size":"1 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197457-CMP08" }, { "size":"1 x 1 Slide", "name":"Human Th1/Th2/Th17 Array (20 T) Glass Slide", "number":"AB197457-CMP04" }, { "size":"1 x 25 µL", "name":"Human Th1/Th2/Th17 Array (20 T) Biotinylated Antibody Cocktail", "number":"AB197457-CMP03" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197457-CMP07" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197457-CMP06" }, { "size":"1 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197457-CMP02" } ] }, "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab197457-50Test", "assetComponentDetails": [ { "size":"4 x 1 Unit", "name":"Adhesive Film", "number":"AB197457-CMP09", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Slide Washer/Dryer (30 mL Centrifuge Tube)", "number":"AB197457-CMP02", "productcode":"" }, { "size":"6 x 30 mL", "name":"20X Wash Buffer I", "number":"AB197457-CMP06", "productcode":"" }, { "size":"4 x 25 µL", "name":"Human Th1/Th2/Th17 Array (20 T) Biotinylated Antibody Cocktail", "number":"AB197457-CMP03", "productcode":"" }, { "size":"4 x 5 µL", "name":"Cy3 equivalent dye-conjugated Streptavidin", "number":"AB197457-CMP08", "productcode":"" }, { "size":"4 x 1 Slide", "name":"Human Th1/Th2/Th17 Array (20 T) Glass Slide", "number":"AB197457-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Th1/Th2/Th17 Array (20 T) Lyophilized Cytokine Standard Mix", "number":"AB197457-CMP05", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer II", "number":"AB197457-CMP07", "productcode":"" }, { "size":"2 x 15 mL", "name":"Sample Diluent", "number":"AB197457-CMP01", "productcode":"" } ] } } }
style
left-column

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
style
left-column
style
left-column

产品实验方案

style
left-column

靶点信息

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 11035086, PubMed : 11352563, PubMed : 20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed : 21376174). CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed : 11352563, PubMed : 9038201). Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity). C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed : 11035086). Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed : 23765988, PubMed : 25122636). Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed : 9129037). May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity). Possesses antibacterial activity towards E.coli ATCC 25922 and S.aureus ATCC 29213 (PubMed : 12149255).
See full target information CCL20

其他靶点

CSF2,IFNG,IFNL2,IL10,IL12A,IL13,IL17A,IL17F,IL1B,IL2,IL21,IL22,IL23A,IL4,IL5,IL6,LTA,TGFB1,TNF
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

搭配使用产品

Multiplex Kits

AB197415

Human Obesity Antibody Array (10 Targets) - Quantitative

Multiplex Protein Detection - Human Obesity Antibody Array (10 Targets) - Quantitative (AB197415)

  • 0
  • 0
Multiplex Kits

AB169809

Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets)

ELISA - Human Th1/Th2/Th17 Antibody Array - Membrane (34 targets) (AB169809)

  • 0
  • 0
Multiplex Kits

AB197456

Human Th1/Th2 Antibody Array (10 Targets) - Quantitative

Multiplex Protein Detection - Human Th1/Th2 Antibody Array (10 Targets) - Quantitative (AB197456)

  • 0
  • 0
Multiplex Kits

AB133998

Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 80 Targets) (AB133998)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com